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| 野生保护抚育杠板归药材的品质评价研究 | |
| 引用本文： | 田莉,陈华国,周欣,谭济苍,薛静. 野生保护抚育杠板归药材的品质评价研究[J]. 中国中药杂志, 2012, 37(9): 1180-1183 |
| 作者姓名： | 田莉 陈华国 周欣 谭济苍 薛静 |
| 作者单位： | 1. 贵州师范大学天然药物质量控制研究中心,贵州贵阳550001;贵州师范大学化学与材料科学学院,贵州贵阳550001 2. 贵州师范大学天然药物质量控制研究中心,贵州贵阳550001;贵州省山地环境信息系统与生态环境保护重点实验室,贵州贵阳550001 3. 贵州远程制药有限责任公司,贵州贵阳,550002 |
| 基金项目： | 国家自然科学基金项目( 81060340);国家科技支撑计划项目(2009BAI74B02-4);贵州省科技创新人才团队建设项目 ;贵阳市科学技术计划项目([2010]筑科计合同字第3-重-1号,[2010]筑科成合同字第1-创27号);贵阳市科技计划大学生科技创业项目([2011] 09);贵州省中药现代化科技产业研究开发专项项目(黔科合社字[2009]5019号) |
| 摘 要： | 目的:采用HPLC测定不同年份贵州省野生护育杠板归药材的指纹图谱,期望能够有效地评价其质量,为杠板归药材的GAP种植提供科学的依据。方法:色谱柱Lichrospher C18(4.6 mm×250 mm,5μm)柱,流速1 mL.min-1,检测波长为340 nm,以乙腈-0.2%醋酸溶液为流动相梯度洗脱。结果:2011年所测产地的杠板归药材比2010年的药材更趋于一致,品质更好;其中大方县的药材在这2年内是最稳定的,贵定县样品GBG-(GD)002和龙里县样品GBG-(LL)003来源地种植的杠板归药材相对其他来源地品质更稳定,基地生产更成熟。结论:建立HPLC指纹图谱的方法来评价各产地杠板归药材的质量与稳定性是可行的,能够从整体上全面而有效地评价杠板归药材的质量,为杠板归药材的GAP种植提供一定的科学依据。 |
| 关 键 词： | 野生抚育 杠板归 指纹图谱 品质评价 |
| 收稿时间： | 2011-12-28 |
Quality evaluation of wildlife tending Polygonum perfoliatum |
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| TIAN Li,CHEN Huaguo,ZHOU Xin,TAN Jicang and XUE Jing. Quality evaluation of wildlife tending Polygonum perfoliatum[J]. China Journal of Chinese Materia Medica, 2012, 37(9): 1180-1183 | |
| Authors: | TIAN Li CHEN Huaguo ZHOU Xin TAN Jicang XUE Jing |
| Affiliation: | The Research Center Quality Control of Natural Medicine, Guizhou Normal University, Guiyang 550001, China; Chemical and Material Science College, Guizhou Normal University, Guiyang 550001, China;The Research Center Quality Control of Natural Medicine, Guizhou Normal University, Guiyang 550001, China; Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guiyang 550001, China;The Research Center Quality Control of Natural Medicine, Guizhou Normal University, Guiyang 550001, China; Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guiyang 550001, China;Yuancheng Pharmaceutical Company, Guiyang 550002, China;Yuancheng Pharmaceutical Company, Guiyang 550002, China |
| Abstract: | Objective: To evaluate the quality of Polygonum perfoliatum collected from GAP planting base efficiently so as to provide scientific evidence for its GAP tending plant by HPLC fingerprinting method.Method: Analysis was performed on a reserved-phase column Lichrosher-C18 column(4.6 mm×250 mm,5 μm),A linear gradient elution of 0.2% aqueous acetic acid and acetonitrile was used for the separation.Result: Wildlife tending P.perfoliatum was relatively stable.The chromatograms of the samples from 2011 were more accordant than those from 2010.Samples in Dafang were the most stable and had little differences during the two years.The quality of P.perfoliatum planted in the regions of sample GBG-(GD) 002 in Guiding and sample GBG-(LL) 003 in Longli were more stable and better than those from other regions.Conclusion: It is a feasible method that can be used to evaluate the quality of P.perfoliatum for GAP planting. |
| Keywords: | wildlife tending Polygonum perfoliatum fingerprinting method quality evaluation |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |