当归黄芪醇提物对实验性皮肤瘙痒模型的药效学研究

目的:观察当归黄芪醇提物(AAE)对实验性皮肤瘙痒模型的影响.方法:AAE 5.0,10.0,20.0 g·kg-1 ig连续7d(小鼠),AAE 3.6,7.2,14.4 g·kg-1 ig连续7d(豚鼠),用右旋糖酐诱发小鼠皮肤瘙痒模型,测定小鼠搔痒潜伏时间及搔痒次数；用磷酸组胺诱发豚鼠皮肤瘙痒模型,测定豚鼠致痒阈；用二甲苯致小鼠耳廓肿胀,测定肿胀度和抑制率；用磷酸组胺致小鼠毛细血管通透性增高,测定风团面积和伊文思蓝渗出量,综合评价AAE对实验性皮肤瘙痒模型的影响.结果:AAE高、中、低剂量均能减少右旋糖酐所致小鼠的搔痒次数及搔痒发作的潜伏时间(P ＜0.05,P＜0.01)；AAE高、中剂量对磷酸组胺所致豚鼠的局部瘙痒有明显的抑制作用(P ＜0.05,P＜0.01)；AAE高、中剂量组小鼠耳廓肿胀度明显减小(P ＜0.05,P＜0.01)；AAE高、中剂量组小鼠腹部风团面积明显减小(P ＜0.05,P＜0.01),伊文思蓝吸光度明显降低(P ＜0.05,P＜0.01).结论:AAE具有显著止痒、抗组织胺、抗炎作用,对实验性皮肤瘙痒具有一定的治疗作用.

Pharmacodynamic Research of Angelica Astragalus Ethanol-extraction on Experimental Itchy Skin Model

Objective:To observe the affection of angelica astragalus ethanol-extraction(AAE) on experimental itchy skin model.Method:The incubation period and the times of itch in mice pruritus model induced by dextran,and the itching threshold in guinea pig induced by phosphoric acid histamine were researched.The inflammatory edema of ear induced by xylol in mice and the increase in mice’s capillary permeability by histamine phosphate were also investigated.The effects of AAE(5.0,10.0,20.0 g·kg-1,ig.for 7 d in mice;8.6,7.2,14.4 g·kg-1 ig,for 7 d in guinea pigs) on all of this models were investigated comprehensively.Result:All dosages of AAE could decrease the times of itch and prolong the incubation period in mices,high and middle doses of AAE obviously inhibit the local itching of guinea pigs and decrease the ear swelling degree or the area of urticaria and the Evans blue absorbance also.Conclusion:There would be antipruritic,antipruritic and anti inflammatory effect of AAE.It has therapeutic effect on experimental pruritus.