丹苘软胶囊对非酒精性脂肪肝模型大鼠胰岛素抵抗及瘦素抵抗的影响

目的:观察丹苘软胶囊对非酒精性脂肪肝( NAFLD)模型大鼠胰岛素抵抗及瘦素抵抗的影响.方法:将大鼠随机分为正常组,模型组,丹苘软胶囊高、中、低剂量组,易善复组,采用高脂饲料建立SD大鼠非酒精性脂肪肝模型,丹苘软胶囊各组分别予相当于丹参石油醚浸膏0.456,0.228,0.114 g·kg-1ig,易善复组每天予易善复0.159 g·kg-1 ig,正常对照组、模型组给药相同体积(1.2 mL·kg-1)的蒸馏水ig.给药4周后测血糖,ELISA法检测血清空腹胰岛素(FINS)、瘦素水平,免疫组化染色法检测肝脏瘦素受体(ob-R)蛋白表达,计算胰岛素抵抗指数(HOMA-IR).结果:空白组、中药各剂量组及西药组血清空腹血糖( FGB),FINS浓度及HOMA-IR,明显低于模型组(P＜0.01,P＜0.05)；空白组、中药各剂量组及西药组血清瘦素水平、肝脏ob-R蛋白表达,明显低于模型组(P＜0.01,P＜0.05).结论:丹苘软胶囊具有改善NAFLD大鼠IR及瘦素抵抗的作用.

Effects of Danqing Soft Capsule on Insulin Resistance and Leptin Resistance in Rats with Non-alcoholic Fatty Liver Disease

Objective :To observe the effects of Danqing soft capsule (DSC) on insulin resistance and leptin resistance in rats with non-alcoholic fatty liver disease (NAFLD). Method :SD rats were randomly divided into control group,model group, DSC high dose group, medium dose group, low dose group, and polyene phosphatidyl choline (PPC) group. rat non-alcoholic fatty liver model was established with high fat diet.DSC high dose group, medium dose group and low dose group were administrated with different concentrations, which are respectively equivalent to petroleum ether extract of salvia 0.456,0.228,0.114 g ·kg^-1. PPC group was treated with PPC by 0.159 g ·kg^-1ig.Control group and model group were given the same volume (1.2 mL ·kg^-1) of distilled water ig. After treatment for 4 weeks, blood glucose was measured; levels of serum insulin and leptin were assayed by ELISA assay; expression of leptin receptor in the liver was observed by immunohistochemical staining; the index of insulin resistance was calculated. Result : Concentrations of FGB, FINS and HOMA-IR in control group, TCM groups and western medicine group were significantly lower than those of model group (P<0.01, P<0.05); the levels of leptin in serum and expression of ob-R protein in liver of control group, TCM groups and western medicine group were significantly lower than those of model group (P<0.01, P<0.05). Conclusion :DSC can improve IR and leptin resistance in rats with NAFLD.