莪术醇对人胚肺成纤维细胞增殖的影响

目的:探讨莪术醇对人胚肺成纤维细胞增殖抑制作用.方法:MTT法观察莪术醇对人胚肺成纤维细胞增殖的抑制作用；天狼猩红染色法测定细胞胶原的分泌能力,流式细胞术检测细胞周期时相分布.结果:50～200 mg·L-1莪术醇作用细胞后能抑制人胚肺成纤维细胞的增殖和制胶原沉积,以96 h效果最为明显,并阻滞细胞停滞于G0/G1期,降低S期和G2/M期细胞时相比例.结论:莪术醇通过将细胞周期阻滞于G0/G1,减少DNA复制,抑制人胚肺成纤维细胞增殖和细胞分泌胶原.

Inhibiting Proliferation of Human Embro Fibroblasts by Curcumol

Objective: To explore the inhibiting the proliferation and the mechanism of human embro fibroblasts (MRC-5) by curcumol. Method: The effects of curcumol on the proliferation of MRC-5 were determined by MTT assay. The cotents of collagen accumulated were determined by the optical density at 540 nm of picro-sirius red staining. The cell cyclic retardation was examined by using flowcytometry. Result: MTT showed that curcumol(50-200 mg ·L^-1) had an inhibitory effect on the proliferation and had reduced collagen of secretion in MRC-5. The better time in the 96 hour. The cell cycle analysia showed that curcumol(50-200 mg ·L^-1) increased the percentage of G₀/G₁ phase cells, updown the percentage of S and G₂/m phase cells. Conclusion :Curcumol can increase the percentage of G₀/G₁ phase cells,delay cell replication, inhibit the proliferation and collagen of secretion of MRC-5.