下瘀血汤全药与组分处方抗猪血清肝纤维化的比较研究

目的:比较下瘀血汤全药与组分处方抗肝纤维化的作用差异.方法:Wistar大鼠随机分为正常组、造模组.造模组大鼠ip猪血清复制肝纤维化模型,每次0.5 mL/只,每周2次,共12周.造模成功后造模组大鼠随机分为模型组、下瘀血汤组、组分处方组,第8周开始下瘀血汤组、组分处方组大鼠造模同时ig给药,按10 mL·kg-1容积,下瘀血汤组每日2.34 g·kg -1,组分处方组每日0.648 g·kg-1.连续4周.正常组、模型组大鼠ig同体积生理盐水.12周末,处死动物,获取标本,检测血清肝功能、肝脏病理.结果:与模型组相比,下瘀血汤组、组分处方组大鼠肝质量明显减少(P＜0.05)；肝体比、脾质量、脾体比均有下降趋势；总蛋白(TP),球蛋白( Glb)含量明显减少(P＜0.05),A/G比值倒置情况明显改善(P＜0.05)；白蛋白(Alb)含量均有升高趋势.下瘀血汤组与组分处方组相比较,大鼠肝质量、肝体比、脾质量、脾体比均有好转趋势；Alb含量有升高趋势,TP,Glb含量有下降趋势,A/G比值倒置情况有改善趋势.与正常组相比,模型组、下瘀血汤组、组分处方组纤维化分级差异有显著性(P＜0.05)；与模型组相比,下瘀血汤组、组分处方组纤维化分级差异有显著性(P＜0.05).提示二者均有一定改善肝功能、改善肝脏病理作用,且下瘀血汤组有优于组分处方组的趋势.结论:降低免疫反应和保护肝细胞是下瘀血汤全药和组分处方抗肝纤维化的主要作用机制,且下瘀血汤全药处方作用优于组分处方.

Comparative Study of Xiayuxue Decoction and Its Ingredients Recipe Against Porcine Serum Induced Liver Fibrosis in Rats

Objective :To compare differences between Xiayuxue decoction and its ingredients recipe in inhibiting hepatic fibrosis. Method: Rat liver fibrosis model was duplicated with intraperitoneal injection of porcine serum. After 8 weeks of modeling, Xiayuxue decoction and its ingredients were administrated for 4 weeks, and at the end of 12 weeks, rats were sacrificed to obtain samples for determination of liver function and examination of liver histology. Result: Compared with model group, improvement in liver function and liver pathology were found in Xiayuxue diction group; Liver function and histology in Xiayuxue dection group was superior to that in its ingredients recipe group. Conclusion: The main action mechanism of Xiayuxue decoction and its ingredients recipe against liver fibrosis is in inhibiting immune response and protecting liver cells, and Xiayuxue decoction is superior to its ingredient recipe.