二至丸保肝活性成分群对CCl4致小鼠急性肝损伤保护作用研究

目的:研究二至丸的保肝活性成分群(50%乙醇组分)(active ingredients group in liver protection from Erzhi Wan,AIEP)对四氯化碳(CCl4)致小鼠急性肝损伤的保护作用。方法:将60只昆明种小鼠随机分成6组,正常对照组、四氯化碳损伤模型组、AIEP的高、中、低剂量组(19.8,13.2,6.6 g.kg-1)和联苯双酯组(150 mg.kg-1)。AIEP高、中、低剂量组每日ig1次,共7 d,末次ig后除正常组外,其余所有小鼠予ip 0.1%CCl420 mL.kg-11次,16 h后处死全部小鼠,收集肝组织及血清标本,测定小鼠血清谷丙转氨酶(ALT)和谷草转氨酶(AST)活性;测定肝匀浆中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活力;计算肝指数并同时对肝组织进行病理学检查。结果:AIEP高、中剂量组具有明显的剂量依赖性降低CCl4致小鼠肝损伤血清ALT,AST升高(P<0.01),降低肝匀浆中MDA的含量(P<0.01),增强SOD的活性(P<0.01)。通过病理学切片观察,AIEP各剂量组能显著改善肝组织的病理变化(P<0.05,P<0.01)。结论:AIEP对CCl4造成的小鼠急性肝损伤具有显著的保护作用,为其保肝活性的效应部位。

Experimental study of active ingredients group in liver protection from Erzhi Wan on acute hepatic injury induced by CCl4 in mice

Objective: To study the active ingredients in liver protection from Erzhi Wan(AIEP) on acute hepatic injury induced by carbon tetrachloride(CCl₄)in mice. Method: Sixty Kunming mice were randomly divided into six groups: the normal group,the model group,bifendate group (150 mg·kg^-1),high AIEP group (19.8 g·kg^-1),middle AIEP group (13.2 g·kg^-1) and low AIEP group (6.6 g·kg^-1).The treatment groups were orally administered once per day for 7 d separately, whereas the normal and model groups were orally administered with saline. Except normal rats,all the other rats were injected intraperitoneally CCl₄ 20 mL·kg^-1 once.The rats were sacrificed 16 h after CCl₄ administration. Serum and liver samples were collected for analysis. The acute hepatic injury model was prepared by CCl₄ injected intraperitoneally. Then,the therapeutic effects of AIEP on the model were evaluated by the activity determination of serum alanine aminotransferase and aspirate aminotransferase (ALT and AST),superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in liver,and the hepatic pathohistological changes following the treatment. Result: The activities of ALT and AST and the MDA content in liver was significantly increased and the activity of SOD was largely inhibited in the animals of modeling group. Following the treatment with AIEP,ALT and AST activities and MDA content were significantly reduced and SOD activity was obviously increased in the mice of treatment group.Furthermore, AIEP could ameliorate the hepatic pathological changes. Conclusion: AIEP have protective effects on acute hepatic injury induced by CCl₄ in mice,and are the effect of the liver protecting active sites.