星点设计-效应面法优化超高压提取红景天中红景天苷

目的:优选超高压提取法提取红景天中红景天苷的最佳工艺.方法:采用星点设计结合效应面法对红景天中红景天苷的超高压提取的工艺参数进行优选,并与回流提取、超声提取和微波提取进行比较.结果:超高压提取红景天中红景天苷的最佳工艺条件为73.3％乙醇,压力255.5 MPa,液固比29.5( mL∶g),提取时间2 min,提取1次；此提取条件下红景天苷的提取率为9.29 mg·g-1,分别高出回流提取31.6％、超声提取20.3％和微波提取9.7％.结论:超高压提取作为一种新兴的中药提取技术,具有提取率高、提取温度低、耗时短、能耗少等优点,为红景天苷提取提供一个全新的方法.

Optimization of Ultrahigh Pressure Extraction for Salidroside From Rhodiola crenulata by Central Composite Design/Response Surface Methodology

Objective:To optimize ultrahigh pressure extraction(UPE) technology of salidroside from Rhodiola crenulata by central composite design/response surface methodology.Method:Central composite design was adopted for getting optimal ultrahigh pressure technique parameters,and compared with conventional extraction’s(reflux extraction,ultrasonic extraction,microwave extraction).Result:Optimal technology conditions of UPE for salidroside from R.crenulata were:solvent was 73.3% ethanol,ratio of solvent to material(mL:g) was 29.5,extraction pressure was 255.5 mPa,extracted 1 time with 2 min.Using this extracting process,extraction yield of salidroside was 9.29 mg·g-1.It was 31.6% higher than reflux extraction,20.3% to ultrasonic extraction and 9.7% to microwave extraction.Conclusion:As a novel extraction technology of Chinese herbal medicine,UPE procedure had higher extraction yield,lower extracting temperature,required a shorter time and was less power consumption.It had provided a brand-novel method for extraction of salidroside from R.crenulata.