胡颓子叶水溶性化学成分的研究

目的:研究胡颓子叶水溶性部位的化学成分.方法:采用大孔树脂柱色谱,反相硅胶柱色谱,SePhadex LH-20柱色谱和半制备高效液相色谱分离纯化,通过理化性质和波谱鉴定化合物结构.结果:从胡颓子叶中分离出2个化合物,其结构分别鉴定为山柰酚-3-O-β-D-葡萄糖基-(1→3) -α-L-鼠李糖基-(1→6)-β-D-半乳糖苷[kaempferol 3-O-β-D-glucopyranosyl-(1→3) -α-L-rhamnopyranosyl-(1→6)-β-D-galactopyranoside,1],山柰酚-3-O-β-D-葡萄糖基-(1→3)-α-L-鼠李糖基-(1→6)-β-D-半乳糖苷-7-O-β-D-葡萄糖苷[ kaempferol 3-O-β-D-glucopyranosyl-(1 →3) -α-L-rhamnopyranosyl-(1→6) -β-D-galactopyranoside-7 -O-β-D-glucopyranoside,2].结论:从胡颓子叶中分离到的化合物2是一个新化合物.

Water-soluble chemical constituents from Elaeagnus pungens leaves

Objective: To study water-soluble chemical constituents from the leaves of Elaeagnus pungens.Method: Chemical constituents of E.pungens leaves were separated by a combination of macroporous resin column chromatography,reverse phase silica gel column chromatography,Sephadex LH-20 column chromatography and semi-preparative HPLC.Their structures were identified on the basis of physicochemical properties using the spectral method.Result: The two compounds were separated from E.pungens leaves and identified as kaempferol 3-O-β-D-glucopyranosyl-(1→3)-α-L-rhamn-opyranosyl-(1→6)-β-D-galactopyranoside(1),kaempferol 3-O-β-D-glucopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside-7-O-β-D-glucopyranoside(2).Conclusion: Compound 2 separated from E.pungens leaves was a new compound.