肾衰养真胶囊对慢性肾衰竭营养不良大鼠下丘脑神经肽Y mRNA及原阿片黑皮素mRNA表达的影响

目的 观察肾衰养真胶囊对慢性肾衰竭 (CRF) 营养不良大鼠下丘脑神经肽 Y (NPY) mRNA 及原阿片黑皮素 (POMC) mRNA 的表达及其营养状态的影响。方法 采用含 0.5% 腺嘌呤及 4% 酪蛋白饲料联合喂养的方法制作 CRF 营养不良大鼠模型，观察其营养不良发生时间，符合 CRF 营养不良模型的随机分为模型组、肾衰养真胶囊组和开同组。治疗4周后，检测下丘脑 NPY mRNA 和 POMC mRNA 表达水平。结果 肾衰养真胶囊组大鼠营养状况明显改善，下丘脑的 NPY mRNA 表达上调及 POMC mRNA 表达下调。结论 肾衰养真胶囊可改善 CRF 营养不良大鼠的营养状况，其机制可能是通过上调下丘脑 NPY mRNA 表达，下调下丘脑 POMC mRNA 表达，从而促进大鼠摄食。

Effects of Shenshuai Yangzhen Capsula on expression of neuropeptide Y mRNA and proopiomelanocortin mRNA in hypothalamus in malnutrition rats with chronic renal failure

Objective To investigate the malnutrition situation in rats induced by adenine and effect of Shenshuai Yangzhen (SSYZ) Capsula, a preparation of Chinese materia medica, on expression of neuropeptide Y (NPY) mRNA and proopiomelanocortin (POMC) mRNA in the hypothalamus in malnutrition rats with chronic renal failure (CRF). Methods SD Rats were fed with 0.5% adenine and 4% casein to make malnutrition in CRF models. Those rats with malnutrition of CRF condition were randomly divided into CRF control group, Ketosteril group, and SSYZ Capsula group. After four weeks treatment, as indicated, the NPY mRNA and POMC mRNA expression level in the hypothalamus of rats were measured by RT-PCR. Results Malnutrition situation in CRF rats began changing at the end of eight week. Compared with those in the CRF group, the malnutrition situation of SSYZ Capsula group was significantly improved. The POMC mRNA expression in SSYZ Capsula group was significantly down-regulated, while the NPY mRNA expression was up-regulated. Conclusion SSYZ Capsula could improve the malnutrition in rats with renal insufficiency possibly by up-regulation of NPY mRNA expression and down-regulation of POMC mRNA expression in the hypothalamus, so as to make rats more food-intake.