仙虎合剂对大鼠实验性脂肪肝治疗作用的研究

目的:观察仙虎合剂对大鼠实验性脂肪肝的治疗作用.方法:Wistar大白鼠50只,随机分为正常对照组、模型组、中药仙虎合剂高、低剂量组和甲硫氨酸组.除正常对照组外,其余4组均以高脂饮食喂养10周,建立高脂血症性脂肪肝模型后,模型组用生理盐水ig,中药高、低剂量组用仙虎合剂(相当于生药31.5,15.75 g·kg-1)ig,甲硫氨酸组用甲硫氨酸片(0.625 g·kg -1) ig.各组于用药4周后处死取材送检,观察肝体比、血脂、肝脂及肝组织病理改变等.结果:模型组镜下呈重度脂肪肝改变,血清总胆固醇(TC)、甘油三酯(TG)分别为(3.09 ±0.84),(2.29±0.41) mmol·L-1,肝组织匀浆TC,TG分别为(5.87±0.65),(23.46±3.28) mmol·L -1,与正常对照组比较有显著性差异(P＜0.01);仙虎合剂高剂量组镜下接近于正常表现,血清TC,TG分别为(1.68 ±0.22),(1.73±0.19)mmol· L-1,肝组织匀浆TC,TG分别为(3.42±0.44),(16.47±2.52)mmol·L -1,与模型组比较有显著性差异(P＜0.01);仙虎合剂高、低剂量组血清TC( 1.68±0.22),(1.72±0.36)mmol·L-1,与甲硫氨酸组( 2.14±0.53) mmol·L-1比较有显著性差异(P＜0.05);3个治疗组其他各项数据相近,提示仙虎合剂能明显降低脂肪肝大鼠血脂,减轻肝脂变及肝细胞坏死程度.结论:仙虎合剂对高脂饮食诱导的脂肪肝具有确切治疗作用.

Effect of Xianhu Mistura on Experimental Liver Steatosis in Rats

Objective: To observe the effect of Xianhu mistura on experimental liver steatosis in rats. Method: Wistar rats were divided into normal group, model group, herbal therapy high dose group, herbal therapy low dose group, methionine group. In addition to normal group, other four groups were fed high fat diet for 10 weeks to established hyperlipemia model. Then the model group was given normal saline gavage, herbal therapy high dose group was given Xianhu mistura (31.5 g ·kg^-1) gavage, low dose group was given Feiluotong mistura (15.75 g ·kg^-1) gavage. Methionine group given methionine tablets gavage. Four weeks later blood biochemistry and pathological changes were examined to observe the effct of Xianhu mistura on common conditions,blood lipid level,enzyme,the ratio of liver to body weight and histological change of the fatty liver rats. Result: Xianhu mistura could decrease blood lipid level and the degree of hepatic and hepatocell necrosis. There was signifcant differences compared with DL-methionine group (P<0.05 or 0.01). Conclusion: Xianhu mistura has better effect in the treatment of hyperlipidemia fatty liver in rats.