复方芳香植物提取物对营养型肥胖大鼠的减肥作用

目的:研究一种复方芳香植物提取物对营养型肥胖大鼠的减肥作用.方法:制备了复方芳香植物水提取物和营养型肥胖大鼠模型,用提取物连续ig给药5周,测量体重、体脂,计算肥胖指数和Lee's指数,ELISA检测血清瘦素(LEP),制作石蜡切片观察脂肪组织形态变化,测定血清超氧化物岐化酶(SOD)、丙二醛(MDA)、总抗氧化能力(T-AOC),初步分析药物作用机制.结果:与营养型肥胖大鼠模型组比较,复方芳香植物提取物各剂量组(4 000,2 000,500 mg· kg-1)大鼠体重和体脂均显著减轻(P＜0.05),肥胖指数和Lee's指数也均有所降低,高剂量组肥胖指数显著降低(P＜0.05)；提取物各剂量组均显著降低大鼠血清LEP水平(P＜0.01或P＜0.05),呈现剂量依赖性；切片观察高剂量组脂肪细胞显著变小(P＜0.01),单位视野细胞数目显著增加(P＜0.01)；各剂量组均显著增加大鼠血清SOD和T-AOC含量、降低MDA含量(P＜0.01),呈现剂量依耐性.结论:本复方芳香植物提取物对营养型肥胖大鼠具有明显的减肥功效和良好的抗氧化作用.

Antiobesity Effects of Water Extract from Compound Aromatic Plants on Diet-induced Obese Rats

Objective:To study the antiobesity function of water extract from compound aromatic plants on diet-induced obese rats.Method:The model of diet-induced obese rats was prepared by feeding high-fat diet for five weeks,then three different doses(4 000,2 000,500 mg·kg-1) of the extract were administered respectively by gavage for another five weeks.The body weight,body fat,obesity index and Lee’s index were measured.The serum activities of leptin(LEP),superoxide dismutase(SOD),malondialdehyde(MDA) and total antioxidant capacity(T-AOC) were assayed by reliable reagent kits.Adipose tissue paraffin sections were examined under a light microscope.The pharmacodynamic mechanism of the extract was substantially analyzed.Result:All doses(4 000,2 000,500 mg·kg-1)of the extract could significantly decrease body weight and body fat of obese rats(P<0.05),and the high dose performed best with obesity index remarkably decreased(P<0.05).All doses of the extract could significantly decrease the concentration of serum LEP(P<0.05),with a dose-dependent manner.The size of lipocytes of the high dose became smaller while the quantity of which in unit microscopic field gets larger markedly(P<0.01).All doses could significantly increase the serum activities of SOD and T-AOC,and decrease MDA levels(P<0.01),with a dose-dependent manner.Conclusion:The water extract from the compound aromatic plants in this study functions well on weight control of diet-induced obese rats,and demonstrates distinctively anti-oxidation activity,which shows promising value of further application.