| 星点设计-效应面法优化升麻提取工艺 |
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| 引用本文： | 王秋红,苏阳,吴伦,王知斌,杨炳友,匡海学. 星点设计-效应面法优化升麻提取工艺[J]. 中国实验方剂学杂志, 2012, 18(1): 24-27 |
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| 作者姓名： | 王秋红 苏阳 吴伦 王知斌 杨炳友 匡海学 |
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| 作者单位： | 黑龙江中医药大学北药基础与应用研究省部共建重点实验室,黑龙江省中药及天然药物药效物质基础研究重点实验室,哈尔滨 150040 |
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| 基金项目： | 黑龙江省创新研究群体科学基金项目(YJSCX2011-429HLT) |
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| 摘 要： | 目的:采用星点设计-效应面法优选升麻中总皂苷的提取工艺.方法:以乙醇体积分数、提取时间、溶媒比为自变量,以升麻总皂苷得率为因变量,通过对自变量各水平的多元线性回归及二项式拟合,用效应面法选取最佳工艺,同时进行预测分析.结果:确定最优提取工艺为15倍量65％乙醇提取3次,每次170 min,提取预测值与理论值偏差0.4％,二项式拟合复相关系数r =0.988 4.结论:星点设计-效应面法优化的升麻总皂苷提取工艺,方法简单,精密度高,可预测性较优.
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| 关 键 词： | 升麻 星点设计 效应面优化法 总皂苷 提取工艺 |
| 收稿时间： | 2011-09-06 |
Optimization of Extraction Technology for Cimicifuga dahurica by Central Composite Design/Response Surface Methodology |
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| WANG Qiu-hong,SU Yang,WU Lun,WANG Zhi-bin,YANG Bing-you and KUANG Hai-xue. Optimization of Extraction Technology for Cimicifuga dahurica by Central Composite Design/Response Surface Methodology[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2012, 18(1): 24-27 |
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| Authors: | WANG Qiu-hong SU Yang WU Lun WANG Zhi-bin YANG Bing-you KUANG Hai-xue |
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| Affiliation: | Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Key Laboratory of Traditional Chinese Medicine and Natural Medicine Pharmacodynamic Material Base, Harbin 150040, China;Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Key Laboratory of Traditional Chinese Medicine and Natural Medicine Pharmacodynamic Material Base, Harbin 150040, China;Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Key Laboratory of Traditional Chinese Medicine and Natural Medicine Pharmacodynamic Material Base, Harbin 150040, China;Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Key Laboratory of Traditional Chinese Medicine and Natural Medicine Pharmacodynamic Material Base, Harbin 150040, China;Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Key Laboratory of Traditional Chinese Medicine and Natural Medicine Pharmacodynamic Material Base, Harbin 150040, China;Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Key Laboratory of Traditional Chinese Medicine and Natural Medicine Pharmacodynamic Material Base, Harbin 150040, China |
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| Abstract: | Objective: To optimize extraction process of total saponins from Cimicifuga dahurica by central composite design/response surface methodology. Method: Independent variables were ethanol concentration, reflux time and solvent fold,dependent variable was extraction rate of total saponins from C. dahurica.Level of independent variables were regressed by multiple linear and fitted by binomial.Response surface methodology was used to optimize extraction process. Prediction was carried out by comparing observed and predicted values. Result: Optimal extraction technology was:extracted 3 times by 15 times the amount of 65% ethanol with 170 min each time,deviation between observed and predicted values was 0.4%. Regression coefficients of binomial fitting complex model was as high as 0.988 4. Conclusion: This extraction process of total saponins from C. dahurica by central composite design/response surface methodology was a simple method with high precision and good predictability. |
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| Keywords: | Cimicifuga dahurica central composite design response surface methodology total saponins extraction process |
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