莪术、三氧化二砷对晶状体上皮细胞增殖及信号转导分子的影响

目的：探讨莪术(RC)、三氧化二砷(As₂O₃)对晶状体上皮细胞(LEC)增殖及细胞信号转导的影响,为防治后发性白内障提供实验依据。方法：采用重组人碱性成纤维细胞生长因子(rhbFGF)诱导牛LEC增殖；四甲基偶氮唑蓝法(MTT)检测RC,As₂O₃对LEC增殖的抑制率；流式细胞术(FCM)检测RC,As₂O₃对LEC增殖细胞核抗原(PCNA)蛋白表达的抑制作用；荧光分光光度法检测LEC内［Ca²⁺］i、放射免疫分析法检测cAMP和cGMP。结果：RC,As₂O₃对rhbFGF诱导的LEC增殖的抑制率增加(P＜0.01)；明显下调rhbFGF诱导增殖的LEC内PCNA蛋白表达(P＜0.01)；使增殖的LEC内［Ca²⁺］i和cAMP显著增高(P＜0.01),cGMP显著降低(P＜0.01)。上述指标均呈剂量-效应关系。结论：RC,As₂O₃能显著抑制LEC增殖；Ca²⁺,cAMP和cGMP细胞信号转导是其抑制LEC增殖的重要分子机制；RC,As₂O₃对防治后发性白内障具有广阔的前景。

Effect of Rhizoma Curcumae and arsenite trioxide on proliferation and signal transduction molecule of lens epithelial cell

Objective: To investigate the effect of Rhizoma Curcumae(RC),arsenite trioxide(As2O3) on proliferation ana signal transduction molecule in lens epithelial cell(LEC),in order to provide experiment evidence for prevention and treatment of after cataract.Method: Proliferation of cultured bovine LEC were induced by induced by recombinant human basic fibroblast growth factor(rhbFGF);Inhibitory rates of LEC proliferation induced by RC,As2O3 were detected by methyl thiazolyl tetrazolium(MTT);Inhibitory effects of expression of proliferating cell nuclear antigen(PCNA) induced by RC,As2O3 in LEC were assayed via flow cytometer(FCM);Concentrations of LEC calcium(i) were determined by spectrofluoremeter,intracellular concentrations of cyclic adenosine monophosphate(cAMP) and cyclic guanosine monophosphate(cGMP) of LEC were measured by radioimmunoassay.Result: Inhibitory rates of RC,As2O3 on LEC proliferation induced by rhbFGF increased significantly,showing dose-dependent(P<0.01).PCNA expression of LEC proliferation induced by rhbFGF were down regulated obviously by RC,As2O3,showing dose-dependent(P<0.01).Concentrations of i and cAMP increased and cGMP decreased significantly in LEC of proliferation inhibited by RC,As2O3(P<0.01).Conclusion: RC,As2O3 can inhibit LEC proliferation obviously.Signal transductions of i,cAMP,cGMP may be the important molecular mechanism.There are broad prospect for RC,As2O3 on prevention and treatment of after cataract.