小檗碱对糖尿病肾病大鼠C反应蛋白的影响

目的:观察小檗碱对糖尿病肾病大鼠C反应蛋白的影响。方法:将48只雄性SD大鼠随机分为空白对照组、模型组、小檗碱75、150及300mg.kg-1组、罗格列酮组六组,每组8只。除正常对照组外,其余各组大鼠腹腔注射链脲佐菌素(STZ,55mg.kg-1),成功造模后小檗碱各组及罗格列酮组分别灌胃给予75、150、300mg.kg-1小檗碱以及4mg.kg-1罗格列酮,连续6w后检测大鼠血糖、体重,HE染色检测肾脏病理改变,ELISA法检测C反应蛋白活性。结果:与正常对照组相比,糖尿病组血糖与C反应蛋白活性明显升高(P<0.05),与模型组比较,小檗碱各组血糖与C反应蛋白活性明显降低(P<0.05),有统计学意义。结论:小檗碱可降低糖尿病大鼠血糖和C反应蛋白,减轻糖尿病肾病。

Effects of berberine on C-reactive protein expression in diabetic nephropathy rats

Objective:To study the effects of berberine on C-reactive protein expression in diabetic nephropathy rats.Methods: 48 male SD rats were divided into model group,control group,rosiglitazone group,berberine 75、150、300 mg·kg~-1 groups randomly,n=8.Diabetes was induced by intraperitoneal injection with 55 mg·kg~-1 streptozotocin(STZ).4 mg·kg~-1 rosiglitazone and 75、150、300 mg·kg~-1 berberine were administrated orally for 6 weeks.The blood glucose,body weight were measured 6 weeks later.Pathological changes in the kidney were measured by hematoxylin and eosin stain,content of C-reactive protein was detected by ELISA.Results: Compared with normal group,C-reactive protein and blood glucose level were increased obviously in model group(P<0.05);compared with model group,C-reactive protein and blood glucose level were decreased obviously in berberine groups(P<0.05).Conclusion: Berberine could decrease the blood sugar level,and this effect is relatived with decreasing the expression of C-reactive protein.