53例急性白血病的免疫表型分析

目的 探讨急性白血病（AL）的免疫表型特征。方法 采用流式细胞仪对53例初治急性白血病进行免疫表型分析。结果 20例急性髓细胞白血病（AML）患者均表达两种以上髓系抗原，其中7例伴有淋系抗原（CD19、CD7）表达。32例急性淋巴细胞白血病（ALL）患者中28例表达淋系抗原，16例表达伴有髓系抗原（CD13、CD33、CD15）；4例ALL患者为纯髓系表达（ALL23例，ALL31例）。2例混合型白血病（AMLL）同时表达两种以上淋系和髓系抗原标志。结论 白血病细胞具有高度异质性；免疫分型能确诊一些特殊类型的白血病（Mo，AMLL），在急性白血病的诊断分型、治疗、预后判断具有重要价值；免疫分型是对细胞形态学诊断急性白血病的重要补充。

Analysis of Immunophenotyping in Acute Leukemia in 53 Cases

Objective To study the feature of immunophenotype in acute Leukemia (AL). Methods Immune phenotypes were assayed in 53 patients with acute Leukemia by flow cytometer. Results All 20 AML patients expressed myeloid markers, of 7 expressed Lymphoid antigens CD7 or/and CD19. Twenty eight of ALL expressed Lymphoid antigens, of 16 expressed one or two myeloid antigens, for example CD13, CD33, CD15. Of 4 ALL cases only expressed myeloid markers (three of them were ALL2,one of them was ALL3).Two cases were hybrid acute leukemia that expressed more markers of both Lymphoid and myeloid origins. Conclusion Leukemia cell has heterogeneity. Leukemia immunophenotype could define some particular types of Leukemia M_0 AMLL with an important value in diagnosis, treatment and predicting prognosis. Immunophenotype is an important supplement to the diagnosis and typing of the acute Leukemia on the base of the morphology and cytochemistry.[JP]