妊娠期糖尿病PAI-1 4G/5G基因多态性与胰岛素抵抗的相关性

目的:探讨纤溶酶原激活物抑制剂-1(PAI-1)4G/5G基因多态性与天津及河北地区汉族人群中妊娠期糖尿病(GDM)发病及胰岛素抵抗(IR)的相关性。方法:用PCR方法检测GDM组和正常妊娠对照组(每组75例)PAI-1 4G/5G基因型,并分析相关临床资料。结果:GDM组4G/4G基因型频率及4G等位基因频率均高于正常对照组(P<0.05)。GDM组4G/4G基因型孕妇空腹胰岛素、胰岛素抵抗指数均高于5G/5G组(P<0.05)。结论:PAI-1 4G/5G基因多态性与GDM发病相关,4G/4G基因型可能是GDM的易感基因;PAI-1 4G/4G基因型与GDM患者IR的发生有关。

The association of PM-14G/5G gene polymorphism with insulin resistance in gestational diabetes mellitus patients

Objective:To investigate the association of PAI-1 4G/5G gene polymorphism with gestational diabetes patients and insulin resistance in Han nationality women of China.Methods:The genotypes of PAI-1 4G/5G variants were determined by polymerase chain reaction(PCR) assay both in women with GDM and pregnant women with normal glucose tolerance (n=75 for each group).Fasting blood glucose,fasting insulin were also analyzed.Results:4G/4G genotype frequency and 4G allele frequency were higher in women with GDM compared with control group(P<0.05).In GDM group,PAI-1 4G/4G genetype-carrying subjects showed higher level of fasting serum insulin,insulin resistance index compared with 5G/5G genetype-carrying subjects (P<0.05).Conclusion:PAI-1 4G/5G polymorphism correlates with the pathogenesis of GDM,suggesting that PAI-1 4G/4G homozygotes is a susceptible gene of GDM.In GDM patiens,PAI-1 4G/4G genetype is one of high risk genetic factors to insulin resistance.