大孔吸附树脂分离纯化葛根总黄酮的研究

 目的研究大孔吸附树脂分离纯化葛根总黄酮的工艺，为葛根总黄酮的工业化生产提供实验依据。方法采用D₁₀₁型、D₂₀₁型、AB-8型3种大孔吸附树脂对葛根总黄酮进行吸附纯化，以总黄酮收率、纯度为考察指标综合评价。结果D₁₀₁型、D₂₀₁型、AB 8型大孔吸附树脂吸附3种方法中静态饱和吸附量以干树脂计分别为113.5，133.1和133.9 mg·g^-1；静态脱吸附率分别为75.6%，66.7%，94.2%；以70%的乙醇为洗脱剂洗脱率最高为92.4%；AB-8型吸附树脂饱和吸附洗脱量为82.0 mg·g^-1；稳定性实验中第11个周期总黄酮收率为91.3%，纯度为86.0%。结论3种纯化方法中，以AB-8型树脂综合性能最好，适合于葛根总黄酮的分离纯化。

Studies on separation and purification of total flavones from Radix Puerariae by macroreticular adsorbents

OBJECTIVE To establish a technical process for the purification of total from flavones Radix Puerariae.METHOD S D₁₀₁、D₂₀₁and AB-8 types of macroreticular adsorbents were used to separate and purify total flavones.The adsorption ratio,the elution ratio,product purity and yield were compared.RESULTS The static adsorption capacity of the three types of macroreticular adsorbents were 113.5，133.1 and 133.9 mg·g^-1 respectively.The static elution ratio were 75.6%,66.7% and 94.2% respectively.The dynamic adsorption capacity of AB 8 type was 82.0 mg·g^-1,and the adsorption ratio was more than 85% after repeating use 11 cycles.CONCLUSION The AB 8 type macroreticular adsorbent showed better comprehensive adsorption property.It can be used to purify the extract of Radix Puerariae.