维生素D受体基因多态性与肺结核易感性的病例对照研究

目的 探讨维生素D受体(VDR)基因多态性与中国汉族人群肺结核发病间的关系。方法 采用病例对照研究设计，用聚合酶链反应—限制性片段长度多态性分析(PCR—RFLP)检测VDR基因中T／C多态性位点，对肺结核相关的环境因素进行问卷调查，并进行单因素分析和多因素1ogistic回归分析。结果 对76例病例和171名对照进行了VDR的基因分型，VDR—FF、VDR—Ff与VDR—ff三种基因型在病例组和对照组中的分布频率分别为38．2％、44．7％、17．1％和52．6％、40．9％、6．4％，其中ff基因型在病例组中的频率显著高于对照组，OR值95％CI：3．668(1．483—9．071)；在多因素分析中调整卡介苗接种史和吸烟状况后，VDR—ff基因型与肺结核发病仍有显著性关联，调整OR值95％CI：3．036(1．117—8．253)。结论 VDR—ff基因型可能是中国汉族人群肺结核病的易感基因型。

A case-control study on the vitamin D receptor gene polymorphisms and susceptibility to pulmonary tuberclosis

Objective To investigate the association between the genetic polymorphisms of VDR gene and susceptibility to pulmonary tuberculosis. Methods Case-control study was conducted. PCR-RFLP technique was used to detect the C/T polymorphism in VDR gene. Information on related factors of tuberculosis was collected using a pre-tested questionnaire. Univariate and multivariate logistic analyses were conducted with SPSS software package. Results A sample of 76 cases and 171 controls was studied. The genotype frequencies of VDR-FF, VDR-Ff and VDR-ff were 38.2 %, 44.7 %, 17.1 % and 52.6 %, 40.9 %, 6.4 % respectively. VDR-ff was significantly overrepresented in case group, the OR (95% CI ) was 3.668 ( 1.483- 9.071 ) when comparing with FF genotype. The significant association remained after adjusting BCG immunization and smoking, the OR (95% CI ) was 3.036 ( 1.117- 8.253 ). Conclusion The VDR-ff genotype might be associated with the susceptibility to pulmonary tuberculosis in Chinese Han population.