Coulter MAXM血细胞分析仪白细胞分类结果的评价

目的　探讨 Coulter MAXM血细胞分析仪对人体血液中白细胞分类计数结果的可靠性。方法　采用 1 5 % EDTA-K2抗凝的静脉全血 1 ml分别用仪器进行白细胞分类计数和制备血涂片经瑞氏染色后在显微镜油镜下分类计数 ,二者结果进行比较分析。结果　该型血细胞分析仪进行白细胞分类计数重复性较好 ,NE,LY,MO,EO,BA等的变异系数分别为1 .5 1 % ,4.5 2 % ,1 0 .7% ,1 6.1 % ,3 0 .1 % ;与显微镜油镜下 2 0 0个白细胞分类计数结果相比 :镜检阴性 1 3 7例中 ,仪器分类有假阳性 5 6例 (占 40 .9% ) ,以显微镜检查分类结果为标准 ,本组病例仪器分类的特异性为 5 9.1 % ,敏感性为 89.5 % ;阴性标本的两种方法白细胞分类计数结果比较 ,中性粒细胞、淋巴细胞及嗜酸粒细胞相差不显著 (P>0 .0 5 )。结论　仪器进行白细胞分类计数和手工显微镜油镜下白细胞分类计数各有所长 ,应联合应用、互相补充。对于白细胞仪器分类计数无警号提示的分析结果可以做为一般性筛查检验 ,可不镜检 ;而对于白细胞分类计数有任何警号提示及确诊为血液病的患者标本均应进行人工显微镜油镜下白细胞分类计数

Evaluation of Differential White Blood CellCounts Taken with the Coulter MAXM Analyzer

Objective To probe the reliability of the differential white blood cell counts in the human blood taken with the Coulter MAXM analyzer Methods We callected 1 ml of 15% EDTA K 2 anticoagulative venous blood, took differential white blood cell count with the analyzer and prepared blood film with it and after Wright's staining took differential white blood cell count under the microscope with oil immersion lens The both results were compared Results The differential white blood cell count taken with the analyzer was better in repetitive tests The variation coefficients of NE, LY, MO, EO, BA were 1.51%, 4.52%, 10.7%, 16.1%, 30.1% respectively To compare the results of differential white blood cell count taken with both methods in the latent samples, there was no marked difference ( P >0.05) in neutrophil, lymphocytes and eosinophilic granulocytes Conclusion Each of the both methods had its advantages, should be applied in combination and complemented with each other