Effects of calcium ions on extracellular and intracellular processes of production of reactive oxygen species in blood phagocytic cells

Preincubation of phagocytes with ryosidine and EGTA increases the intracellular production of reactive oxygen species and decreases their extracellular production in cells activated with phorbol 12-myristate 13-acetate. Ryosidine and EGTA decreased intra-and extracellular production of reactive oxygen species in formyl peptide-stimulated leukocytes. The addition of the ionophore A23187 led to the appearance of reactive oxygen species in the extracellular, but not in the intracellular, medium. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 126, No. 9, pp. 334–337, September, 1998