Alkaline phosphatase activity in chronic streptozotocin-induced insulin deficiency in the rat: Effect of insulin replacement |
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Authors: | Stephen Hough Louis V. Avioli Steven L. Teitelbaum Michael D. Fallon |
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Affiliation: | 1. Division of Bone and Mineral Metabolism, Department of Medicine, The Jewish Hospital of St. Louis, Washington University School of Medicine, St. Louis, Missouri, USA.;2. the Department of Pathology, The Jewish Hospital of St. Louis, Washington University School of Medicine, St. Louis, Missouri, USA.;3. Laboratory Medicine, The Jewish Hospital of St. Louis, Washington University School of Medicine, St. Louis, Missouri, USA. |
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Abstract: | Alterations in circulating alkaline phosphatase have been described in both man and the experimental animal with chronic insulin deficiency. We evaluated plasma and tissue alkaline phosphatase levels in freely-fed control, streptozotocin-induced diabetic and insulin-treated diabetic rats, seven weeks after the induction of diabetes. Circulating alkaline phosphatase activity was markedly elevated in the insulin deficient animal (p < 0.001) and completely normalized following insulin administration. The elevated plasma alkaline phosphatase activity observed in the insulin deficient animals was heat-resistant and phenylalanine-sensitive, a pattern typical of the intestinal isoenzyme. Small intestinal alkaline phosphatase activity was significantly higher (p < 0.01) in the diabetic animals, but comparable in the insulin-replaced and control rats. The intestinal isoenzyme activity was found to be strikingly insulin-sensitive; withholding insulin therapy for 36 hr prior to sacrifice resulted in an abrupt rise in both plasma and intestinal alkaline phosphatase values comparable to those observed in the insulin-deficient state. In contrast to these observations, skeletal alkaline phosphatase activity was decreased in the insulin deficient animal (p < 0.01) and this abnormality was corrected by insulin replacement. Neither insulin deficiency nor insulin replacement resulted in any significant changes in the hepatic alkaline phosphatase isoenzyme. |
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Keywords: | Address reprint requests to Michael D. Fallon M.D. Department of Pathology The Jewish Hospital of St. Louis 216 South Kings Highway St. Louis Missouri 63110. |
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