Fractionation and identification of Euglena gracilis cytoplasmic and chloroplastic tRNAs and mapping of tRNA genes on chloroplast DNA |
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| Authors: | M. Kuntz M. Keller E. J. Crouse G. Burkard J. H. Weil |
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| Affiliation: | (1) Institut de Biologie Moléculaire et Cellulaire du CNRS, Université Louis Pasteur, 15 rue Descartes, 67084 Strasbourg, France |
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| Abstract: | Summary The cytoplasmic and chloroplast tRNAs of Euglena gracilis Z strain were fractionated by two-dimensional gel electrophoresis and identified by aminoacylation. Purified chloroplast tRNAs, labeled in vitro with |32P|, were hybridized to endonuclease restriction fragments of chloroplast DNA, allowing the corresponding tRNA genes to be localized on the physical map of Euglena chloroplast DNA. |
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| Keywords: | Euglena gracilis Z strain tRNAs Chloroplast tRNA genes Hybridization |
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