高效液相色谱法同时测定对乙酰氨基酚和非那西丁的血药浓度

目的:建立以高效液相色谱法同时测定对乙酰氨基酚和非那西丁血药浓度的方法。方法:以DiamonsilC18 为色谱柱,甲醇-水(45∶55)为流动相,茶碱为内标,检测波长为254nm ,柱温为室温。结果:对乙酰氨基酚和非那西丁的最低检测浓度为5μg/ml,线性范围为5～25μg/ml;方法回收率均>95 % ,日内、日间RSD均<4 .0 %。结论:本法简便、准确,能满足临床对于对乙酰氨基酚和非那西丁血药浓度测定的要求。

Simultaneous Determination of Blood Drug Concentration of Acetaminophen and Phenacetin by HPLC

OBJECTIVE:To establish a HPLC method for the simultaneous determination of blood drug concentration of acetaminophen and phenacetin.METHODS:Diamonil C 18 was used as the chromatographic column,the mobile phase was methanol-water(45∶55),theophyllinum was used as internal standard,the detection wavelength was254nm and the column temperature was set at the room temperature.RESULTS:The minimum determination concentration of acetaminophen and phenacetin was5μg/ml,the calibration curve was linear in a range from5～25μg/ml;The recovery rate of both acetaminophen and phenacetin in this method was more than95%;both the intraday RSD and the interday RSD were less than4.0%.CONCLUSION:The method is rapid and accurate,which can satisfy the needs of the clinic blood drug concentration deter-mination of paracetamol and phenacetin.