低温冷冻神经损伤与再生研究

目的通过冷冻大鼠坐骨神经探讨用于低温冷冻神经的变性，功能丧失与再生和功能恢复的实验参数，用于指导冷冻外科的临床治疗。方法阶梯温度冷冻犬鼠坐骨神经，采用病理观察、步态分析及皮层体感诱发电位潜伏期测量等方法，对冷冻致神经损伤、再生和肢体运动、感觉功能恢复作出评定。结果应用-60℃至-100℃冷冻神经可完全阻断神经传导且保持基底膜完整，能保证冷冻神经的完全再生，而低于-100℃的温度将摧毁神经再生的“脚支架”，在-60℃至-100℃温度范围内，采用较高温度冷冻神经后运动及感觉功能恢复较快。结论-60℃至-100℃温度范围可保证神经冷冻效果及神经冷冻后的完全再生。

The study of nerval damage and regenesis after low temperature freezing

Objective To investigate the low temperature freezing effect on nerve regenesis and damage in order to serve the clinic application. Methods The nerves were frozen with different low temperatures. The damaged and regenerated nerve were examined for pathological change, sciatic functional index, somatosensory evoked potential. Results The nerval basement membrane could be kept integrity when the nerve was frozen between - 60 ℃ to - 100 ℃ and the nerve could be regenerated completely. If the temperature was lower than - 100℃, the nerve fibre became demolished. However, the nerve could be regenerated faster when temperature higher than -60℃. Conclusion The nerve can be effectively freezen and regenerated completely in between -60 ℃ to -100℃ temperature range.