MicroRNA-133在急性心肌梗死诊断中的研究

目的：研究了MicroRNA-133（miR-133）在急性心肌梗死急性期的表达情况。方法：本研究纳入急性心肌梗死（AMI）患者38例，对照组患者35例。在心肌梗死症状发作后的不同时间段：AMI患者人院时（T0：5．24h±1．38h），AMI发病24h（T24），AMI后第7天采血液样本并应用实时定量PCR法对血浆中miR-133表达水平进行检测。应用酶联免疫吸附测定（ELISA）法检测血浆肌钙蛋白I（cTnI）的表达情况。结果：急性心肌梗死患者血浆中miR-133的表达水平在AMI患者人院时、24h时均较对照组明显升高（P〈0．05），而第7天时其表达水平与对照组无明显差异（P〉0．05）。AMI患者入院时血浆miR-133表达水平已经达到峰值，发病24h时明显下降且于第7天降至正常水平。AMI早期血浆miR-133与cTnI显示了相同的升高趋势。受试者工作特征曲线（ROC曲线）分析结果显示血浆miR-133的ROC曲线下面积（AUC）在1D与T24时分别为0．802、0．753（所有P〈0．05）。结论：在心肌梗死急性期miR-133在AMI早期表达水平明显升高，而且拥有较之肌钙蛋白酶I（cTnI）更早的时间窗。血浆miR-133在诊断AMI时具有较高的敏感度与特异性，这提示其可能作为急性心肌梗死的早期诊断生物标记物。

Study of MicroRNA-133 in diagnosis of acute myocardial infraction

Objective：To investigate MicroRNA- 133 （miR- 133） expression in acute phase of acute myocardial infarction. Methods ：38 patients with acute myocardial infarction （AMI） and 35 patients in control group were selected. Blood samples were sam- pied when AMI patients were admitted into the hospital （ T0 ： 5.24 h ＋ 1. 38 h）, 24h after AMI incidence （ T24）, and 7 days after AMI incidence; and miR - 133 expression levels in plasma were detected through real - time quantitative PCR. The expression of plas- ma troponin I （cTnI） was detected by enzyme - linked immunosorbent assay （ELISA）. Results ： The expression levels of miR - 133 in the plasma of the patients with acute myocardial infarction at TO and T24 were obviously higher than those in the control group （P 〈 0. 05 ）, while difference of the expression levels between the two groups was not significant 7 days after AMI incidence （ P 〉 0.05 ）. For AMI patients, the plasma miR - 133 expression levels had reached to the peak value at TO, obviously decreased at T24, and reduced to normal level 7 days after AMI incidence. Plasma CTnI and miR - 133 in the AMI early stage showed the same rising trend. Receiver operating characteristic （ROC） curve analysis results showed that areas under the ROC curve （AUC） of plasma miR- 133 at TO and T24 were 0.802 and 0.753, respectively （ all P 〈 0.05 ）. Conclusions： MiR - 133 expression levels in the acute phase of myocardial infarction is significantly higher and is earlier than troponin enzyme I （cTnI） in time window. Plasma miR -133 has a high sensitivity and specificity in the diagnosis of AMI, suggesting it can be used as a biomarker for early diagnosis of acute myocardial infarction.