| 重组人粒细胞集落刺激因子对HL-60细胞影响的研究 |
| |
| 引用本文： | 徐文华,王鈜,孙立荣.重组人粒细胞集落刺激因子对HL-60细胞影响的研究[J].医学分子生物学杂志,2005,2(3):178-180. |
| |
| 作者姓名： | 徐文华 王鈜 孙立荣 |
| |
| 作者单位： | 青岛大学医学院生物化学与分子生物学教研室,青岛市,266021;青岛大学附属医院儿科,青岛市,266003 |
| |
| 摘 要： | 目的研究重组人粒细胞集落刺激因子(rhG-CSF)对HL-60细胞的影响。方法瑞氏染色观察细胞形态的变化,吖啶橙/溴化乙锭双染色观察凋亡情况,NBT和CD13测定观察成熟分化情况,MTT实验观察增殖活性。结果加入rhG-CSF 48 h后,HL-60细胞凋亡率升高,增殖活性下降,而且随着rhG-CSF浓度的升高,这种作用更加明显。成熟分化特征变化不明显。结论rhG-CSF有促进HL-60细胞凋亡并抑制其增殖的作用。
|
| 关 键 词： | 重组人粒细胞集落刺激因子 凋亡 HL-60细胞系 |
| 修稿时间： | 2005年1月6日 |
Effects of Human Recombinant Granulocyte-colony Stimulating Factor on HL-60 Cell |
| |
| Xu Wenhua,WANG Hong,Sun Lirong.Effects of Human Recombinant Granulocyte-colony Stimulating Factor on HL-60 Cell[J].Journal of Medical Molecular Biology,2005,2(3):178-180. |
| |
| Authors: | Xu Wenhua WANG Hong Sun Lirong |
| |
| Institution: | XU Wenhua~1,WANG Hong~2,SUN Lirong~2 1 Department of Biochemistry and Molecular Biology,Qingdao Medical College,Qingdao University,Qingdao,266021,China 2 Department of Pediatrics,the Affiliated Hospital of Qingdao University,Qindao,266003,China |
| |
| Abstract: | Objective To investigate the effects of rhG-CSF on HL-60 cells. Methods HL-60 cells were cultured in different concentrations of rhG-CSF, and the cells were morphologically observed by using Wrights, acridine orange (AO) and ethidium bromide (EB) staining, MTT assay, nitroblue tetrazolium (NBT), expression of CD13. Results Apoptosis of HL-60 cells was enhanced and proliferation was inhibited after culture with rhG-CSF for 48 hours, and the enhancing and inhibiting effects were stronger with the rise of rhG-CSF concentration. Conclusion RhG-CSF could enhance the apoptosis of HL-60 cell and inhibit the proliferation. |
| |
| Keywords: | recombinant human granulocyte-clony simulating factor apoptosis HL-60 cell line |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
