Enzyme-linked immunosorbent assay to detect hepatitis C virus serological groups 1 to 6 |
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Authors: | Mariko Kobayashi Kazuaki Chayama Yasuji Arase Akihito Tsubota Satoshi Saitoh Yoshiyuki Suzuki Masahiro Kobayashi Kenji Ikeda Marie Matsuda Hiromi Koike Michie Hashimoto Hiromitsu Kumada |
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Institution: | (1) Department of Liver Research Laboratory, Toranomon Hospital, 1-3-1 Kajigaya, Takatsu-ku, Kawasaki 213-8587, Japan, JP;(2) Department of Gastroenterology, Toranomon Hospital, Tokyo, Japan, JP |
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Abstract: | By conventional serological grouping methods, it is possible to determine hepatitis C virus (HCV) serological groups for
genotypes 1a, and 1b, and genotypes 2a, and 2b, but not for other genotypes, i.e., 3a, 3b, 4a, 5a, and 6a. In this study,
we attempted to serologically group HCV with the Murex HCV serotyping 1 to 6 assay (Murex Diagnostics, Kent, UK), using an
enzyme-linked immunosorbent assay (ELISA) based on genotype-specific peptides from the NS4 region. The subjects of this study
were 365 patients infected with HCV of genotype 1a, 1b, 2a, 2b, 3a, or 3b. The sensitivity of the assay was 100% in patients
with genotype 1a, 82.7% in those with 1b, 68.5% in those with 2a, 84.2% in those with 2b, 50.0% in those with 3a, and 76.5%
in those with genotype 3b. The overall sensitivity was 78.4%. The specificity of the assay was 100% in the subjects with genotype
1a, 98.8% in those with 1b, 98.4% in those with 2a, 96.9% in those with 2b, 100% in those with 3a, and 100% in those with
genotype 3b. The overall specificity was 98.6%. The concordance of the assay was 100% in subjects with genotype 1a, 81.7%
in those with 1b, 67.4% in those with 2a, 81.6% in those with 2b, 50.0% in those with 3a, and 76.5% in those with genotype
3b. The overall concordance was 77.5%. We believe it would be better to serotype with the Murex HCV serotyping 1 to 6 assay,
if other than serological group (Gr) 1 or Gr 2 is suspected in particular ethnic groups or in subjects with an indeterminate
result with the Immucheck HCV Gr assay (Kokusai, Kobe, Japan), assuming that the genotype must be other than 1a, 1b, 2a, or
2b.
Received: October 22, 1998 / Accepted: February 26, 1999 |
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Keywords: | : HCV serological group HCV serotype HCV |
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