肋间神经移植加NGF、BDNF基因修饰的OECs修复大鼠脊髓损伤

目的探讨自体肋间神经移植加NGF、BDNF基因修饰的嗅神经鞘细胞（OECs）修复大鼠脊髓损伤（Spinal cord injury，SCI）。方法SD大鼠72只，经T11、T12间隙平面切取2mm的半侧脊髓，制成半切伤模型。动物分为A、B、C三组，每组24只。A组：将自体肋间神经纤维损伤脊髓近端前角灰质与远端前索白质桥接连接，伤侧L1、L2、L3、L4脊神经节与近端后索白质及远端后角灰质与近端后索白质桥接连接，并植入NGF、BDNF基因修饰的OECs；B组：同上述方法移植肋间神经，不植入OECs；C组：单纯脊髓损伤，脊髓半切缺损处填充明胶海棉，术后4、8周对各组动物进行神经功能检查（斜板实验检查，BBB评分），感觉诱发电位（SEP）和运动诱发电位（MEP）检测以及组织学检查。结果术后4周时，A、B组和动物神经功能，斜板试验，BBB评分及SFP、MEP检测结果均优于C组，8周时，A组动物神经功能，斜板试验，BBB评分，SEP、MEP检测结果均优于B组和C组，镜下见移植组织存活，充填于缺损处，部分脊髓组织融合，结论肋间神经移植加NGF、BDNF基因修饰的OECs对SCI的修复有一定的疗效。

The Intercostal Nerve Transplantation with Olfactory Ensheathing Cells Modified by NGF, BDNF Genes Repairs the Injured Rat Spinal Cord

Objective To study the repairable effects of intercostal nerve transplantation with NGF,BDNF genes modifying olfactory Ensheathing cells(OECs) on spinal cord injury(SCI).Methods The spinal cord hemisection model was made by cutting 2 mm rat spinal cord from T_(11) and T_(12).And then the lesion was repaired using intercostal nerve linking manner(proximal gray substance to distal white substance and L_1,L_2,L_3,L_4 of radix posterior and proximal white substance),and with NGF,BDNF genes modifying OECs were transplanted into group A.However,the group B was only transplanted to get the intercostal nerve but no OECs,the group C was without treatment but an absorbable gelatin sponge was used to SCI.The operated model rat had been bred for 4 to 8 weeks.The BBB scores were checked,and the somatosensory evoked potentials(SEP),motor evoked potentials(MEP) were examined,and the tissue structure of the operated region was observed under the light microscope.Results The 8 weeks of after operation,the back limbs function of group A rats was recovered,and BBB scores were A>B>C,and the peak latencies of early waves in SEP and MEP were A