细胞外基质金属蛋白酶诱导因子和血管内皮生长因子在骨巨细胞瘤中的表达及临床意义

目的：研究骨巨细胞瘤（GC T ）中细胞外基质金属蛋白酶诱导因子（EM M PRIN ）-CD147、血管内皮生长因子（VEGF）的表达情况，探析两者间的相关性及与临床病理、预后、复发的关系，为临床判断GCT 生物学行为及评估预后提供准确的检验指标，也为临床治疗提供新的思路。方法选择2010年2至2013年4月隆昌县人民医院行手术治疗的GCT患者病理切片68份，采用免疫组织化学法（即S-P法）检测CD147、VEGF、基质金属蛋白酶（M M P）等分子表达情况，分析两者的相关性及患者病理资料、Jaffe′s分级、预后的影响因素。结果患者性别、年龄等一般资料与CD147、VEGF阳性表达无相关；各级Jaffe′s分级患者CD147和MMP-2阳性表达差异有统计学意义（P＜0．05），预后各分组患者CD147、MMP-2、MMP-9阳性表达差异有统计学意义（P＜0．05）；CD147和MMP-9、V EA F的表达呈正相关（ r＝0．696，P＝0．025；r＝0．674，P＝0．000）。结论 CD147的阳性表达和GC T 患者病情恶化以及复发有着密切的关系，并且和MMP-9、VEGF的阳性表达密切相关，是目前临床评价GCT恶性程度以及评估预后的重要检测指标。

Expression and clinical significance of CD147 and VEGF in giant cell tumor of bone

Objective To study the expression status of extracellular matrix metalloproteinases （MMP） indu-cing factor （CD147） and vascular endothelial growth factor （VEGF） in giant cell tumor （GCT） of bone ,to explore the correlation between the two factors and their relations with clinical pathology ,prognosis and recurrence of disea-ses for accurate judgment of disease′s biological behaviors and prognosis assessment .Methods Immune histochemi-cal method （S-P method） was used to detect expression levels of CD147 ,VEGF and MMP genes in pathological sec-tions of 68 patients with GCT of bone ,who receiving surgical treatment during February 2010 to April 2010 .The cor-relation between CD147 and VEGF ,and the influence factors on pathological characters ,Jaffe′s classification and prognosis .Results Gender and age of patients were not related to positive expression of CD 147 and VEGF .The difference of positive expression of CD147 and MMP-2 between patients with Jaffe′s classification were significant （P＆lt;0 .05） .The differences of positive expression of CD147 ,MMP-2 and MMP-9 in patients with different prognosis were significant （P＆lt;0 .05） .The expression of CD147 was positively correlated to MMP-9（r=0 .696 ,P=0 .025） and VEGF （r=0 .674 ,P=0 .000） .Conclusion The positive expression of CD147 could be closely related to the disease deterioration and recurrence of GCT of bone ,and related to positive expression of MMP-9 and VEGF .CD147 might be an effective indicator for evaluation of malignant degree and prognosis of GCT of bone .