17β-雌二醇对小鼠淋巴结、脾脏和胸腺调节性T细胞及其Foxp3表达的影响

目的：研究小鼠去卵巢及体内注射17β-雌二醇（E2）后，淋巴结、脾脏和胸腺CD4^＋CD25^＋调节性T细胞（regulatory Tcell，Treg细胞）及相关调节性因子Foxp3表达的变化与E2的相关性。方法：采用流式细胞仪检测CD4^＋CD25^＋Treg细胞功能相关因子Foxp3的表达，CD4^＋CD25^＋T和CD4^＋CD25^＋high T细胞数量的变化。结果：E2抑制淋巴结中Treg细胞Foxp3的表达．上调其在脾脏和胸腺中的表达；E2下调淋巴结中CD4^＋CD25^＋T细胞和CD4^＋CD25^＋high T细胞的比例，上调它们在脾脏尤其是胸腺中的比例。结论：E2对淋巴结、脾脏和胸腺3种免疫器官中的Treg细胞有不同的调节作用。

Different effect of 17β-estradiol on Treg cells and Foxp3 expression of lymph node,spleen and thymus in mouse

Objective To investigate the effect of 17β-estradiol（E2） on CD4^＋ CD25^＋ Treg cells and the expression of their function relevance factor Foxp3 in the lymph nodes, spleen and thymus in vivo. Methods The expression of Foxp3 and the proportion of CD4^＋ CD25^＋ T/CD4^＋ CD25^＋ high T ceils were detected by FCM. Results E2 could inhibit the expression of Foxp3 in lymph node, while upregulate it in spleen and thymus;E2 could downregulate the proportion of CD4^＋ CD25^＋ T/CD4^＋ CD25^＋high T ceils in lymph nodes,but upregulate them in spleen and thymus,especially in spleen. Conclusion E2 has different effect on Treg ceils in lymph nodes, spleen and thymus.