人BAFF-R-IgG4mut融合蛋白对BXSB狼疮鼠CD4~+T细胞增殖活性的影响 |
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引用本文: | 丁涵露,王莉,倪兵,高闻达,钟雪梅. 人BAFF-R-IgG4mut融合蛋白对BXSB狼疮鼠CD4~+T细胞增殖活性的影响[J]. 四川医学, 2011, 32(8): 1157-1160 |
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作者姓名: | 丁涵露 王莉 倪兵 高闻达 钟雪梅 |
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作者单位: | 1. 四川省人民医院肾内科,四川,成都,610072 2. 第三军医大学免疫学研究所,重庆,400038 3. 哈佛大学Beth Israel Deaconess医学中心免疫研究所,美国,波士顿 4. 波士顿大学医学中心,美国,波士顿 |
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基金项目: | 四川省卫生厅课题资助项目(编号:303005002239032) |
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摘 要: | 目的观察人BAFF-R-IgG4mut融合蛋白对BXSB狼疮鼠CD4+T淋巴细胞增殖活性的影响。方法体外分离、纯化BXSB狼疮鼠脾脏CD4+T细胞,流式细胞技术检测CD4+T细胞BAFF-R表达,MTT法观察不同剂量BAFF-R-IgG4mut融合蛋白对BAFF刺激的CD4+T细胞增殖活性的影响。结果台盼蓝染色分离后的BXSB狼疮鼠脾脏CD4+T细胞活性率为(93.6±3.2)%;流式细胞技术检测BXSB狼疮鼠脾脏CD4+T细胞表达BAFF-R;100ng/ml、200ng/ml、400ng/ml BAFF-R-IgG4mut蛋白作用于BAFF和CD3抗体刺激的CD4+T细胞后,CD4+T细胞增殖抑制率分别是56.24%、67.07%和75.45%,较单纯BAFF+CD3抗体组差异有统计学意义(P〈0.05)。结论 BAFF-R-IgG4mut融合蛋白可体外抑制BXSB狼疮鼠CD4+T细胞增殖活性。
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关 键 词: | B细胞刺激因子受体 B细胞刺激因子 BAFF-R-IgG4mut融合蛋白 CD4+T淋巴细胞 |
The effect of BAFF-R-IgG4mut fusion protein on proliferative activity of CD4~+T lymphocytes in murine BXSB lupus |
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Affiliation: | DING Han-lu1,WANG Li1,NI Bing2,et al.1.The People′s Hospita of Sichuan,Chengdu,Sichuan 610072;2.The Immunology Institute,The Third Military Medical University,Chongqing 400038,China |
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Abstract: | Objective Study the effect of human BAFF-R-IgG4mut fusion protein on proliferative activity of CD4+T lymphocytes in murine BXSB lupus.Methods Isolation and purification of BXSB lupus mouse spleen CD4+T cells.BAFF-R expression in CD4+T cells was detected by flow cytometry.The effect of different doses of BAFF-R-IgG4mut fusion protein on the proliferative activity of CD4+T cell under BAFF and CD3 antibody stimulation were assessed by MTT.Results Activity of CD4+T cells separatiing from BXSB lupus mouse were(9... |
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Keywords: | BAFF-R BAFF BAFF-R-IgG4mut fusion protein CD4 T lymphocytes |
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