| HPLC测定降脂酮中槐角苷的含量 |
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| 引用本文： | 郑行林,罗森,袁崇均,王笳,陈帅. HPLC测定降脂酮中槐角苷的含量[J]. 四川中医, 2011, 0(6): 46-47 |
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| 作者姓名： | 郑行林 罗森 袁崇均 王笳 陈帅 |
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| 作者单位： | 成都中医药大学附属医院药剂科;四川省中医药科学院; |
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| 摘 要： | 目的：建立降脂酮中槐角苷的含量测定方法。方法：色谱柱为Kromasil C18柱（4.6 mm×150 mm,5μm）,柱温：28℃,流动相为甲醇-水（60∶40）,流速1ml/min,检测波长为260nm。结果：槐角苷在0.01~0.20μg内峰面积与进样量呈良好的线性关系,Y=20063X＋9.1712（r=0.9998）,平均回收率为99.51%,RSD为0.85%（n=6）。结论：方法简便、准确、重现性好,可作为控制产品质量的定量依据。
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| 关 键 词： | 降脂酮 槐角苷 高效液相色谱法 |
Determining the Content of Saponin in JiangZhiTong by HPLC |
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| Zheng Xinglin Luo Sen,Yuan Chongjun Wang Jia Chen Shuai. Determining the Content of Saponin in JiangZhiTong by HPLC[J]. Journal of Sichuan of Traditional Medicine, 2011, 0(6): 46-47 |
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| Authors: | Zheng Xinglin Luo Sen Yuan Chongjun Wang Jia Chen Shuai |
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| Affiliation: | Zheng Xinglin1 Luo Sen2,Yuan Chongjun2 Wang Jia2 Chen Shuai21.Teaching Hospital Pharmacy of Chengdu University of Traditional Chinese Medicine(Sichuan Chengdu 610072,China) 2.Sichuan Academy Of Chinese Medicine Sciences(Sichuan Chengdu 610041,China) |
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| Abstract: | Objective：To establish an HPLC method to determine the content of Saponin in JiangZhiTong.Methods：Kromasil C18 column（150mm ×4.6mm,5μm）was used with a mobile phase of methanol – water（60 ∶40） and the flow rate was 1.0ml·min-1,the detection wavelength was at 260nm and temperature at 28℃.Results：Saponin presented a good linear correlation in the range of 0.01～0.20μg（r = 0.9998）.The average recovery was 99.51% and RSD was 0.85%.Conclusion：The method is convenient,accurate and reproducible.It can be used for quality control of this preparation. |
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| Keywords: | JiangZhiTong Saponia HPLC |
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