输血传播性病毒的流行病学及临床研究

目的:观察分析不同人群中TTV(transfusion transmitted virus)感染状况及相关临床意义。方法:在TTVORF1设计引物,建立巢式聚合酶链反应,检测不同人群中血清TTV DNA,并对比观察肝炎病人的临床表现。结果:29例健康人群,27例职业献血员,56例乙型肝炎,31例丙型肝炎和47例非甲～非庚型肝炎患者中,TTV DNA阳性率分别为6.9％、3.7％、23.2％、25.8％和42.6％。3种肝炎病人中,TTV DNA阳性和阴性组间4项主要临床指标无显著差异。结论:健康人群和职业献血员存在TTV健康携带者。非甲～非庚型肝炎患者TTV感染率最高。乙型和丙型肝炎患者重叠TTV感染较常见。3种肝炎病人中,合并TTV感染组与非感染组临床表现无明显差异。TTV的致病性尚待深入研究。

Epidemiological and Clinical Study on TTV Infection among Various Populations

Aim: To investigate transfusion transmitted virus (TTV) infection in various populations and its clinical significance. Methods: A nested polymerase chain reaction (PCR) assay with primers from ORF1 of TTV genome was established and it was used to detect TTV DNA in the serum samples from various populations. The patients with TTV DNA positive or not were comparatively and clinically observed. Results: TTV DNA was detected in the sera from 2 of 29 (6.9%) general individuals, 1 of 27 (3.7%) professional blood donors, 13 of 56 (23.2%) patients with hepatitis B, 8 of 31 (25. 8%) patients with hepatitis C and 20 of 47 (42. 6%) patients with non A-non G hepatitis. The four major clinical indexes between groups with TTV DNA positive or negative had no significant difference. Conclusions: The results show that TTV infection is common in the general individuals and blood donors; TTV infection rate was the highest in patients with non A-non G hepatitis; The patients with hepatitis B and C were commonly infected with TTV. But the pathogenesis of TTV needs to be further investigated.