| PCR杂交梳技术在乙型肝炎诊断中的应用 |
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| 引用本文： | 郭劲松,曾令兰.PCR杂交梳技术在乙型肝炎诊断中的应用[J].中西医结合肝病杂志,1999,9(3):8-9. |
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| 作者姓名： | 郭劲松 曾令兰 |
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| 作者单位： | 同济医科大学附属协和医院传染病教研室，同济医科大学附属协和医院传染病教研室，同济医科大学附属协和医院传染病教研室，同济医科大学附属协和医院传染病教研室，同济医科大学附属协和医院传染病教研室 武汉 430022，武汉 430022，武汉 430022，武汉 430022，武汉 430022 |
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| 摘 要： | 目的:为临床诊断乙型肝炎提供灵敏、准确、可靠的实验依据。方法:采用PCR杂交梳(反相膜杂交法),对71例乙型肝炎患者血清进行DNA验证性检测,并与一般聚合酶链反应(PCR琼脂糖凝胶电泳法)同步对比分析。结果:71例乙型肝炎患者血清中杂交梳方法检出HBV DNA阳性58例,阳性率81.7％;琼脂糖凝胶电泳质检出HBV DNA阳性50例,阳性率70％。结论:PCR杂交梳法较PCR法具有更高特异性和敏感性。
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| 关 键 词： | 乙型肝炎病毒 杂交梳 聚合酶链反应 |
Application for Detecting Hepatitis B Virus with Hybri-Comb Technique |
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| Guo Jingsong,Zeng Linglan,Li Shuli,et al..Application for Detecting Hepatitis B Virus with Hybri-Comb Technique[J].Chinese Journal of Integrated Traditonal and Western Medicine on Liver Diseases,1999,9(3):8-9. |
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| Authors: | Guo Jingsong Zeng Linglan Li Shuli |
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| Institution: | Guo Jingsong,Zeng Linglan,Li Shuli,et al. Department of Infectious Diseases,Union Hospital,Tongji Mecical Vniversity |
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| Abstract: | Aim:To probe into a more sensitive.more accurate and more specific method to detect HBV DNA. Methods: Serum samples of 71 patients with hepatitis B were detected for HBV DNA by PCR hybri-comb (reversal membrane hybridization), at the same time, usual polymerase chain reaction assay (PCR-electrophoresis in agarose gel) was given as controls. Result: 58 serum samples were positive for HBV DNA by PCR hybri-comb assay among 71 patients samples. Positive ratio was 81. 7% ; however, 50 serum samples were positive by PCR-electrophoresis in agarose gel, and positive ratio was 70%. Conclusion: PCR hybri-comb technique is satisfactory with sensitivity and specificity in detecting HBV DNA. |
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| Keywords: | HBV Hybri-comb Polymerase Chain Reaetion |
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