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| RP-HPLC法测定克痒敏酊中盐酸小檗碱的含量 | |
| 引用本文： | 陈蓉,周洪波,梅全喜.RP-HPLC法测定克痒敏酊中盐酸小檗碱的含量[J].中国药房,2008,19(21):1646-1647. |
| 作者姓名： | 陈蓉 周洪波 梅全喜 |
| 作者单位： | 1. 遵义医学院第五附属珠海医院,珠海市 2. 广东药学院中山校区实验中心,中山市,528405 3. 广州中医药大学附属中山中医院,中山市,528400 |
| 摘 要： | 目的:建立以反相高效液相色谱法测定克痒敏酊中盐酸小檗碱含量的方法。方法:色谱柱为AgilentEclipse XDB C18(250mm×4.6mm,5μm),流动相为乙腈-5mmol.L-1辛烷磺酸钠(32:68),检测波长为346nm,柱温为35℃。结果:盐酸小檗碱进样量在0.0592～0.8883μg范围内与峰面积积分值呈良好线性关系(r=0.9999);平均回收率为99.71%,RSD=1.18%(n=6)。结论:本方法灵敏、准确、重现性好,可用于克痒敏酊的质量控制。 |
| 关 键 词： | 反相高效液相色谱法 盐酸小檗碱 克痒敏酊 含量测定 |
Determination of Berberine Hydrochloride in Keyangmin Tincture by RP - HPLC |
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| Institution: | CHEN Rong,Quan-xi(1.Dept. of Pharmacy, The Fifth Affiliated Zhuhai Hospital of Zunyi Medical College, Zhuhai 519100, China;2.MEI Zhongshan Hospital Affiliated to Guangzhou University of TCM, Zhongshan 528400, China) |
| Abstract: | OBJECTIVE ： To establish an RP - HPLC method for the determination of Berberine hydrochloride in Keyangin tincture.METHODS： The sample separation was performed on Agilent Eclipse XDB C18（250 mm×4.6 mm, 5μm）, and the mobile phase was acetonitrile - 5 mmol × L ^-1 perfluorooctane sulfonate（32 ： 68） at flow rate of 1 mL × min^ -1. The detection wavelength was 346 nm and the temperature of column was 35 ℃ .RESULTS： The linear range of Berberine hydrochloride was 0.059 2--0.888 3μg（ r = 0.999 9） .The average recovery rate was 99.71%（RSD = 1.18%, n = 6）. CONCLUSION： The met hod is sensitive, accurate, and reproducible, and suitable for the determination of Berberine hydrochloride in Keyangmin tincture. |
| Keywords: | RP - HPLC Berberine hydrochloride Keyangmin tincture Content determination |
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