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| 胰岛素可抵抗MPP^＋诱导的PC12细胞的凋亡 | |
| 引用本文： | 郭丽萍,王坚,蒋雨平,宗鸿亮,王秋雁,郭鹏,顾建新.胰岛素可抵抗MPP^＋诱导的PC12细胞的凋亡[J].中国临床神经科学,2004,12(2):143-146,154. |
| 作者姓名： | 郭丽萍 王坚 蒋雨平 宗鸿亮 王秋雁 郭鹏 顾建新 |
| 作者单位： | 1. 山西医科大学第二医院神经内科,030001 2. 复旦大学附属华山医院神经内科,200040 3. 复旦大学上海医学院基因中心、生化教研室,200032 |
| 摘 要： | 目的观察胰岛素在MPP+诱导PC12细胞凋亡中的干预作用.方法应用MTT法研究细胞活性的改变,应用HOECHST33258染色结合荧光显微镜技术及流式细胞技术检测不同药物对PC12细胞的凋亡诱导作用,应用半定量逆转录聚合酶链式反应(RT-PCR)测定胰岛素受体(insulin receptor,IR)mRNA的改变.结果①MPP+诱导PC12细胞凋亡,胰岛素可以抵抗此凋亡作用;②以上两种处理,均未见到胰岛素受体mRNA的改变,推测胰岛素受体的自身磷酸化有改变.结论胰岛素可以抵抗MPP+诱导的PC12细胞的凋亡. |
| 关 键 词： | 胰岛素 胰岛素受体 凋亡 帕金森病 PC12细胞 |
| 文章编号： | 1008-0678(2004)02-0143-04 |
| 修稿时间： | 2004年4月2日 |
Protective Effect of Insulin Against 1-Methyl-4-Phenylpyridine-Induced Apoptosis in PC12 Cells |
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| ?.GUO Li-Ping,WANG Jian,JIANG Yu-Ping,et al.Protective Effect of Insulin Against 1-Methyl-4-Phenylpyridine-Induced Apoptosis in PC12 Cells[J].Chinese Journal of Clinical Neurosciences,2004,12(2):143-146,154. | |
| Authors: | ?GUO Li-Ping WANG Jian JIANG Yu-Ping |
| Institution: | ?.GUO Li-Ping,WANG Jian,JIANG Yu-Ping,et al Department of Neurology,Huashan Hospital,Fudan University,Shanghai 200040,China.2.ZONG Hong-Liang,GU Jian-Xin,WANG Qiu-Yan,GUO Peng State Key Laboratory of Genetic Engineering,Gene Research Center and Department of Biochemistry,Fudan University,Shanghai 200032,China.3.GUO Li-Ping Department of Neurology,the 2nd Hospital,Shanxi Medical University,Shanxi 030001,China |
| Abstract: | Aim:To study the effect of the insulin against 1-methyl-4-phenylpyridium ion (MPP +)induced apoptosis in pheochromocytoma (PC12) cells.Methods:3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay,HOECHST 33258 staining method,flow cytometric analysis were performed on PC 12 cells,the expression of insulin receptor (IR) mRNA was measured by using RT-PCR.Results:(1)Cell treated with MPP + exhibited various apoptotic features,while cells pretreated with insulin prior to MPP + exposure showed a decrease in the occurrence of apoptotic features.(2)Both MPP + and insulin had no effects on the expression of IRmRNA,the phosphorylation of IR may be a potential mechanism.Conclusion:The insulin may exert a protective effect against MPP +-induced apoptosis in PC12 cells. |
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