| Hsp70L1增强肿瘤细胞疫苗免疫原性的研究 |
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| 引用本文： | 尤红煜,张开霞,王俊霞,张焕铃,连伟光,吴为,宋淑霞. Hsp70L1增强肿瘤细胞疫苗免疫原性的研究[J]. 细胞与分子免疫学杂志, 2010, 26(4) |
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| 作者姓名： | 尤红煜 张开霞 王俊霞 张焕铃 连伟光 吴为 宋淑霞 |
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| 作者单位： | 河北医科大学分子生物学研究室,实验动物重点实验室,河北石家庄,050017 |
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| 基金项目： | 河北省科技支撑资助项目(09276418D-10;052761990) |
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| 摘 要： | 目的:考察Hsp70L1对肿瘤细胞免疫原性的增强作用。方法:用RT-PCR的方法,从小鼠黑色素瘤B16细胞和C57BL/6小鼠脾脏中获得TRP2153-243及Hsp70L1基因。分别插入pcDNA3.1/V5-His真核表达载体,构建pHSP70L1、pTRP和pTRP2-Hsp3种表达载体。分别转染B16肿瘤细胞并制备坏死或凋亡瘤苗,免疫C57BL/6小鼠后移植B16肿瘤细胞,观察肿瘤生长曲线,采用流式细胞术(FCM)或微量细胞毒的方法检测荷瘤小鼠细胞因子INF-γ和CTL活性。结果:将经过HSP70L1、TRP2及TRP2-HSP基因修饰的坏死或凋亡的B16肿瘤细胞免疫正常小鼠后,观察到Hsp70L1及TRP2-Hsp基因修饰的坏死瘤苗可显著抑制荷瘤鼠肿瘤的生长,并显著促进荷瘤鼠脾脏淋巴细胞CTL活性和IFN-γ产生(P0.05,P0.01);HSP70L1免疫刺激作用在坏死瘤苗中更明显。结论:Hsp70L1可明显提高B16瘤苗的免疫原性,且对坏死细胞瘤苗的作用更显著。
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| 关 键 词： | Hsp70L1 HSP70L1-TRP2融合基因 细胞坏死/细胞凋亡 佐剂 肿瘤模型 |
Immunoadjuvant effect of Hsp70L1 in tumor vaccine |
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| YOU Hong-yu,ZHANG Kai-xia,WANG Jun-xia,ZHANG Huan-ling,LIAN Wei-guang,WU Wei,SONG Shu-xia. Immunoadjuvant effect of Hsp70L1 in tumor vaccine[J]. Chinese journal of cellular and molecular immunology, 2010, 26(4) |
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| Authors: | YOU Hong-yu ZHANG Kai-xia WANG Jun-xia ZHANG Huan-ling LIAN Wei-guang WU Wei SONG Shu-xia |
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| Abstract: | AIM:To explore the immune enhancement of Hsp70L1 in the tumor cell vaccines. METHODS:TRP2153-243 and Hsp70L1 genes were obtained by RT-PCR from B16 cells in murine melanoma and from spleens of C57BL/6 mice and then were inserted into pcDNA3.1/V5-His eukaryotic expression vectors respectively. The recombinants of pTRP2,pHSP70L1 or pTRP2-Hsp fusion gene were obtained and transfected into B16 cells respectively. TRP2153-243,HSP70L1 or TRP2-Hsp fusion gene-expressing B16 cells were then induced to necrosis by f... |
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| Keywords: | Hsp70L1 Hsp70L1-TRP2 fusion gene cell necrosis/cell apoptosis adjuvant tumor model |
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