聚合酶链式反应检测胆囊结石患者胆汁细菌DNA及意义

目的研究胆囊结石患者胆汁细菌ＤＮＡ，探讨胆结石形成与细菌感染的关系。方法采用聚合酶链式反应（ＰＣＲ），对７０例胆囊结石患者胆汁中５种细菌ＤＮＡ进行研究。结果５种细菌中细菌ＤＮＡ存在５６例，其中单菌ＤＮＡ２９例，双菌ＤＮＡ１９例，多菌ＤＮＡ８例；首次在胆汁中检测出幽门螺杆菌。结论胆结石患者胆汁中存在多种细菌ＤＮＡ；胆结石核心形成可能与细菌感染有关。

Test and significance on bacteria deoxyribonucleic acid of bile in gallstones patients with polymerase chain reaction

Objectives To study bile bacteria deoxyribonucleic acid (DNA) in gallstones patients and to detect relationship between gallstones form and bacteria infection.Methods Bile DNA of bacteria has been studied in 64 gallstones patients with poly-merase chain reaction (PCR).Results DNA of bacteria was 56 patients in five bacteria,on which DNA of simple bacterium,double bactreia and multiple bacteria was 29 patients,19 patients and 10 patients,respectively.DNA of helicobacter pylori was first tested in bile.Conclusions There was DNA of multiple bacteria in bile.Gallstones nuclear form was related to bacteria infection.