大肠癌外周血循环肿瘤细胞数的检测及临床意义

目的 检测大肠癌（CRC）患者外周血循环肿瘤细胞（CTCs）数,探讨其临床意义.方法 58例CRC患者术前静脉采血,使用多重聚合酶链式反应（PCR）结合荧光定量PCR（RTFQ PCR）方法检测外周血细胞角蛋白20（CK20）及癌胚抗原（CEA） mRNA表达,比较CTCs细胞阳性率与临床病理参数的关系.结果 58例CRC患者外周血中CK20及CEAmRNA表达的阳性率为93.1％.CTCs的阳性检出率与Ducke＇s分期及淋巴结转移相关（P＜0.05）.结论 多重RTFQ PCR方法可应用于CRC患者外周血中CTCs检测,对预后判断有着重要的临床意义.

Detection and clinical significance of peripheral blood circulating tumor cells in patients with colorectal cancer

Objective To investigate the positive rate of circulating tumor cells （CTCs） in peripheral blood of patients with colorectal cancer （CRC） and explore its clinical significance.Methods Venous blood samples were collected from 58 CRC patients before surgery.Multiplex-PCR combined with fluorescence quantitative PCR （RTFQ PCR） was applied to detect the cytokeratin 20 （CK20） and carcinoembryonic antigen （CEA） mRNA expressions in peripheral blood.The positive rate of CTCs among patients was compared with clinicopathological parameters.Results The positive rate of CK20 and CEA mRNA expressions were up to 93.1%.The positive rate of CTCs was associated with Ducke＇s staging and lymph node metastasis （P 〈 0.05）.Conclusion Multiplex-RTFQ PCR can be applied to detect the CTCs in peripheral blood in CRC patients and has important clinical significance on prognosis judgment.