| 抗hAR—N段McAb的制备及其在免疫组化检测中的应用 |
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| 引用本文： | 沈敬华 唐振华. 抗hAR—N段McAb的制备及其在免疫组化检测中的应用[J]. 内蒙古医学院学报, 2000, 22(3): 150-152 |
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| 作者姓名： | 沈敬华 唐振华 |
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| 作者单位： | 内蒙古医学院微生物教研室(沈敬华，贾柄权)，内蒙古医学院教务处(唐振华)，内蒙古医学院病理室(赵利珍)，内蒙古医学院医疗系(李维才) |
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| 摘 要： | 目的：自行研制抗人雄激素受体（ａｎｄｒｏｇｅｎ ｒｅｃｅｐｔｏｒ，ＡＲ）单克隆抗体（ＭｃＡｂ），以期达到可替代国外ＡＲ多克隆抗体。方法：采用人雄激素受体合成肽（ｈＡＲ－Ｎ）交联钥孔戚血蓝素（ＫＬＨ）为抗原，免疫ＢＡＬＢ／Ｃ小鼠，经用Ｄｏｈｌｅｒ和Ｍｉｌｓｔｅｉｎ杂交瘤技术成功地获得４株能稳定分泌抗ｈＡＲ单克隆抗体（ＭｃＡｂ）杂交瘤细胞株５５，１４１，２０７，２５７。结果：对４５例前列腺癌组织石蜡切
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| 关 键 词： | 前列腺肿瘤 单克降抗体 免疫组织化学 |
PREPARATION AND APPLICATION OF MONOCLONAL ANTIBODIES AGAINST hAR-N IN IMMUNOHISTOCHEMISTRY |
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| SHEN Jing hua,TANG Zhen hua,ZHAO Li zhen,et al. PREPARATION AND APPLICATION OF MONOCLONAL ANTIBODIES AGAINST hAR-N IN IMMUNOHISTOCHEMISTRY[J]. Acta Academiae Medicinae Neimongol, 2000, 22(3): 150-152 |
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| Authors: | SHEN Jing hua TANG Zhen hua ZHAO Li zhen et al |
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| Abstract: | Objective:To study and prepare anti human androgen receptor(AR) monoclonal antibodies (McAb).Methods:Four cell lines of hybridoma secreting specific McAb against AR were first established by fusion SP2/0 cells with spleen cells from BALB/C mice immunized with the coupling complex of hAR-KLH.Results:Paraffin-embedded sections of 45 prostate cancers were detected.There was an overall concordance of 91% using IHC between AR ployclonal antibody from Zymed and hAR-N McAb in our work.Conclusion:The results show that the McAb obtained in this study would be a useful tool to detect the AR status in prostate cancer. |
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| Keywords: | prostatic neoplasms antibodiy monoclonal receptor androgen imunohistochemistry |
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