石上柏颗粒对肝癌模型小鼠瘤组织中Cyclin D1、CKS表达的影响

目的:研究石上柏颗粒对肝癌模型小鼠瘤组织中Cyclin D1、CKS表达的影响。方法:选取48只肝癌模型小鼠,随机分为为模型组、氟尿嘧啶组、石上柏颗粒组、联合给药组,每组各12例。氟尿嘧啶组给予氟尿嘧啶20 mg·kg~(-1)灌胃,石上柏颗粒组给予石上柏颗粒120 mg·kg~(-1)灌胃,联合给药组同时灌胃氟尿嘧啶20 mg·kg~(-1)和石上柏颗粒120mg·kg~(-1),模型组小鼠给予同体积纯净水,各组小鼠连续灌胃不同药物4周,比较各组小鼠治疗期间肿瘤生长情况,采用RT-PRC法测定各组小鼠瘤组织中Cyclin D1 mRNA、CKS mRNA的表达,采用West-blot法测定瘤组织中Cyclin D1、CKS蛋白的表达。结果:(1)给药1周、2周、3周、4周时氟尿嘧啶组小鼠移植瘤体积均较模型组小鼠显著降低(P0.05),给药2周、3周、4周时石上柏颗粒组小鼠移植瘤体积均较模型组小鼠显著降低(P0.05),给药3周、4周时联合给药组移植瘤体积均较氟尿嘧啶组小鼠显著降低,差异有统计学意义(P0.05);(2)给药4周时氟尿嘧啶组、石上柏颗粒组和联合给药组小鼠瘤组织Cyclin D1 mRNA、CKS mRNA均较模型组显著降低(P0.05),而联合给药组小鼠瘤组织Cyclin D1mRNA、CKS mRNA较氟尿嘧啶组显著降低,差异有统计学意义(P0.05);(3)给药4周时,氟尿嘧啶组、石上柏颗粒组和联合给药组小鼠瘤组织Cyclin D1和CKS蛋白均较模型组显著降低(P0.05),而联合给药组小鼠瘤组织Cyclin D1和CKS蛋白较氟尿嘧啶组显著降低,差异有统计学意义(P0.05)。结论:石上柏颗粒有显著抗肝癌作用,且可以增强氟尿嘧啶的疗效,其抗肝癌作用可能与其抑制Cyclin D1和CKS表达有关。

Effect of Selaginella Granule on Cyclin D1 and CKS Expression in Tumor Tissue of Mice with Hepatocellular Carcinoma

Objective:To study the effect of Selaginella granule on the expression of Cyclin D1 and CKS in tumor tissues of mice with hepatocellular carcinoma.Methods:Forty-eight HCC mice were randomly divided into model group,fluorouracil group,Selaginella granule group and combined group,with 12 cases in each group.The rats in the fluorouracil group were treated with fluorouracil 20 mg · kg-1,and the rats in Selaginella granule group were treated with Selaginella granule 120 mg · kg-1.The rats in the combined group were given both medications.The mice in the model control group were given the same volume of pure water.The mice of each group were treated with different drugs for 4 weeks.The tumor growth was analyzed.The expressions of Cyclin D1 mRNA and CKS mRNA in the tumor tissue of each group were measured with RT-PRC method.The expression of Cyclin D1 and CKS protein in tumor tissue was detected with West-blot.Results:①The volume of transplanted tumor in the fluorouracil group was significantly lower than that in the model group (P ＜ 0.05) at 1 week,2 weeks,3 weeks and 4 weeks (P ＜ 0.05).The volume of transplanted tumor in the Selaginella granule group was significantly lower than that in the fluorouracil group at 3 weeks and 4 weeks(P ＜ 0.05).②The expression of Cyclin D1 mRNA and CKS mRNA in the fluorouracil group,the Selaginella granule group and the combined administration group were significantly lower than those in the model group (P ＜ 0.05).③Four weeks after administration,the levels of Cyclin D1 mRNA and CKS mRNA in the fluorouracil group,the Selaginella granule group and the combined administration group were significantly lower than those in the model group.The expression of Cyclin D1 and CKS in the tumor tissue of the mice was significantly lower than that of the model group(P ＜ 0.05),but the expression of Cyclin D1 and CKS protein in the combined group was significantly lower than that in the fluorouracil group(P ＜ 0.05).Conclusion:Selaginella granule has a significant anti-hepatocarcinoma effect,and it can enhance the efficacy of fluorouracil.Its anti-hepatoma effect may be related to its inhibition the expression of Cyclin D1 and CKS.