高良姜、胡椒和槟榔有效成分对岗田酸诱导p-tau细胞模型的作用研究

目的:探讨高良姜、胡椒和槟榔有效成分对岗田酸诱导p-tau细胞模型的作用。方法:采用CCK8法确定48 h内岗田酸,高良姜素、胡椒碱和槟榔碱对高分化PC12细胞的作用浓度,随之将其分为空白对照组,模型对照组、高良姜素组、胡椒碱组和槟榔碱组,检测细胞中p-tau含量和观察细胞形态变化。结果:在48 h内,岗田酸的造模适应浓度是175 n M,高良姜素、胡椒碱和槟榔碱的LD50分别是150 pM、24 pM和250 pM。与空白对照组比较,模型对照组的p-tau显著增加(P0.01),说明岗田酸诱导p-tau细胞模型造模成功;与模型对照组比较,高良姜素组、胡椒碱组和槟榔碱组的p-tau显著减少(P0.01),细胞形态的损伤减少。结论:高良姜素、胡椒碱和槟榔碱对岗田酸诱导p-tau模型细胞有一定的作用。文献引用:赵贤武,黄丽平,邓敏贞,等.高良姜、胡椒和槟榔有效成分对岗田酸诱导p-tau细胞模型的作用研究[J].中医学报,2017,32(11):2176-2180.

Effect of the Component of Galangal,Pepper and Areca on p-tau cell model induced by Okadaic Acid

Objective:To study the effect of the component of Galangal,Pepper and Areca on p-tau cell model induced by Okadaic Acid (OA).Methods:The concentration of OA,Galangin,Piperine and Arecoline for high differentiated PC12 cell in 48 hours was detected by CCK8 method.Then it can be divided into blank control group,model control group,Galangin treated-group,Piperine treated-group and Arecoline treated-group.P-tau content and morphological changes were tested.Results:The LD50 of OA,Galangin,Piperine and Arecoline within 48 h was 175 nM,150 pM,24 pM,and 250 pM.P-tau in the model control group increased significantly compared with that in the blank control group (P ＜ 0.01).It meaned that p-tau cell model induced by OA was successfully completed.P-tau levels of Galangin treated-group,Piperine treated-group and Arecoline treated-group were significantly decreased (P ＜ 0.01) and cell morphology damage reduced compared with those in the model control group.Conclusion:Galangin,Piperine and Arecoline have effect on p-tau cell model induced by OA.