P53基因突变、人乳头瘤病毒16，18型感染与肺鳞癌发病的关系

应用单链构象多态性分析多聚酶链反应ＥＢ染色法（ＰＣＲ－ＳＳＣＰ－ＥＢ）对４０例肺癌组织标本的Ｐ５３基因序列５～８外显子突变进行分析，同时应用ＰＣＲ方法检测上述标本及１８例肺良性疾病组织标本中ＨＰＶ１６、１８型ＤＮＡ相关序列，旨在分析二者在肺鳞癌发病中的作用及相互关系，并就肺鳞癌与Ｐ５３基因结构和功能异常的关系进行初步探讨。

Analysis of Action of P53 Gene Mutation and Papilloma Virus Type 16 and 18 Infection in Iung Squamous Cell Carcinoma

To study structural and functional aberration of tumor suppressor P53 gene,PCR-SSCP-EB method was used to detect exons 5 to 8 of P53 gene in 40 specimens of lung squamous cell carcinoma;HPV I6 to 18 DNA sequences were examined in the same samples.The results showed that:(1)67.5% of tumor tissues had P53 gene mutations.Mutations of P53 gene were more common in tumors from smokers than nonsmokers(P<0.05).There was no significant difference between the two groups with and without lymph node metastasis. (2)HPV 16 to 18 DNA sequences occurred in 42.5%,the percentage of which was higher than that of the samples from benign pulmonary diseases(P< 0.01).(3)There was an inverse correlation between the presence of HPV 16 to 18 infection and mutations of P53 gene.The results indicated that mutation of P53 gene was an early event in the progression of lung squamous cell carcinoma. HPV 16 to 18 infection may be one of the causes this kind of cancer.