Frequent deletion of the transgene in T cell receptor {beta} chain transgenic mice |
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Authors: | Komori Shinji; Katsumata Makoto; Greene Mark I; Yui Katsuyuki |
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Institution: | Division of Immunology, Department of Pathology and Laboratory Medicine, University of Pennsylvania 36th and Hamilton Walk, Philadelphia, PA 19104–6082, USA
1Present address: Department of Obstetrics and Gynecology, Hyogo Medical College 1-1, Mukogawa-cho, Nishinomiya, 663 Japan |
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Abstract: | TCR Vß8.1 transgenic mice were generated using a genomicTCR Vß gene construct under the control of its promoterand enhancer. Among three lines of transgenic mice, one lineexpressed the transgenic TCR on only 70% of peripheral T cells,while the other two lines expressed it on almost all matureT cells. T cells which lacked expression of the transgenic TCRß chain expressed endogenous TCR ß chains.The molecular basis underlying the lack of transgene expressionin T cells of this line of transgenic mice was investigated.The transgenic TCR– cells were isolated by two methods.First, Thy-1+ Vß8.1/8.2– cells were purifiedfrom peripheral T cells using cell sorting. Second, transgenicTCR– T cell clones were established. In both cases, Southernblotting indicated that Vß8.1– T cells had deletedthe transgenic TCR gene. Thus, deletion of the transgenic TCRcan occur in a high proportion of T cells, which allows rearrangementand expression of endogenous TCR ß chains. |
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Keywords: | rearrangement TCR ß chain transgene deletion |
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