复方宁喘雾化剂纯化工艺研究

目的：为提高药物疗效,减少用药量,研究复方宁喘雾化剂纯化工艺。方法：分别采用化学方法与药效学方法来考察不同的纯化工艺。用过滤,离心及50%、60%、70%醇化5种方法对水煎药液进行纯化。①化学考察：以处方中主要药物麻黄与黄芩中的麻黄碱,伪麻黄碱及黄芩苷为指标,用HPLC法测定并比较指标含量。②药效学考察：分别观察全白豚鼠经5种纯化药液给药后各治疗组（分别为中药雾化1组、中药雾化2组、中药雾化3组、中药雾化4组、中药雾化5组）、模型组、正常组及西药组的肺与支气管组织形态,肺脏器指数。结果：当药液的醇沉浓度达到60%时,麻黄中的麻黄碱与伪麻黄碱总和为3.04 mg/g,黄芩中黄芩苷含量为77.6 mg/g,分别高于其他方法。药效观察结果显示,中药雾化4组（60%醇化）的治疗效果较好。结论：综合以上含量测定和药效学实验的结果,60%醇化为最佳纯化工艺。

Study on Purification Techniques of "Compound Ningchuan Nebulizer"

Objective： To study the purification techniques of ＂ Compound Ningchuan Nebulizer＂ in order to improve the efficacy and reduce the amount of medication. Methods： The chemical and phatraacodynamic methods were applied to investigate the five different purification processes which were filtration, eentrifugation, and 50% , 60% , 70% alcoholization. （1） The chemical method： The contents of ephedrine and pseudocphedrine in Herba Ephedrae and baicalin in Radix Scutellariae were determined and compared by HPLC. （2）The pharmacodynamic method： The white guinea pigs were divided into eight groups which were the five purified liquid medicine groups, the normal group, the model group and the western medicine group. The lung and bronchial tissue morphology and the lung indexes were observed. Results： when the alcohol concentration reached 60%, the sum of ephedrine and pseudoephedrine contents was 3.04 mg · g^- 1 , and the baicalin content was 77.6 mg·g^- 1, which were higher than the other methods. The pharmacodynamic results showed that the effect of the 60% alcoholization group was the best. Conclusion： The best purification processs was 60% aleoholization method.