缺氧／再给氧和细胞因子对白细胞与脑微血管内皮细胞粘附作用？…

目的：研究缺氧／再给氧（Ｈ／Ｒ）对中性粒细胞（Ｎｅｕ）和单核细胞（Ｍｏｎ）与诱导的培养牛脑微血管细胞粘附作用的影响。方法：牛脑微血管内皮细胞（ＣＭＦＣ）和平滑肌细胞（ＣＭＳＭＣ）经Ｈ／Ｒ处理或经ＴＮＦ－α和ＩＬ－１α刺激，Ｍｏｎ和Ｎｅｕ与ＣＭＥＣ和ＣＭＳＭＣ粘附的细胞数目用流式细胞仪测定，结果：ＣＭＥＣ先缺氧２ｈ再经复氧与ＴＮＦ－α（２μｇ．Ｌ＾－１）作用２ｈ，Ｍｏｎ和Ｎｅｕ与ＣＭＥＣ的粘附率分别

Effects of hypoxia/reoxygenation and cytokines on adhesion of leukocytes to cerebral microvascular endothelial cells.

AIM: To study the influence of hypoxia/reoxygenation (H/R) on the adhesion of neutrophils (Neu) and monocytes (Mon) to cultured bovine cerebral microvascular cells induced by tumor necrosis factor alpha (TNF-alpha) or interleukin 1-alpha (IL-1 alpha). METHODS: Bovine cerebral microvascular endothelial cells (CMEC) and smooth muscle cells (CMSMC) were exposed to normoxia or H/R in the presence or absence of TNF-alpha (IL-1 alpha). The number of adhesions of Mon and Neu to CMEC and CMSMC was measured by flow cytometry. RESULTS: CMEC were subjected to hypoxia for 2 h followed by reoxygenation with TNF-alpha (2 micrograms.L-1) for 2 h, the adhesion rate of CMEC for Mon and Neu were increased to 35.0% +/- 0.9% and 36.0% +/- 0.6% (the TNF-alpha treated group was 28.9% +/- 1.1% and 28.8% +/- 1.3%), respectively. CMSMC were treated as above, the adhesion rate of Mon and Neu was enhanced to 49.9% +/- 0.4% and 43.9% +/- 1.4% (the TNF-alpha treated group was 34.0% +/- 1.9% and 34.0% +/- 1.3%), respectively. CMEC and CMSMC were treated by IL-1 alpha, similar results were obtained. CONCLUSION: H/R enhanced the adhesion of Mon and Neu to CMEC and CMSMC induced by TNF-alpha or IL-1 alpha.