重组人纤连蛋白CH50对黑色素瘤生长及转移的影响

目的：研究重组人纤连蛋白ＣＨ５０对小鼠黑色素瘤生长的影响及其抗肿瘤机制。方法 进行小鼠动物实验及黑色素瘤Ｂ１６细胞实验。结果：ＣＨ５０在体内明显抑制黑色素瘤生长及实验性肺转移，ＣＨ５０在体外能附粘黑色素瘤Ｂ１６细胞，抑制Ｂ１６细胞粘附层粘蛋白并明显提高腹腔巨噬细胞对Ｂ１６细胞杀伤活性，结论：ＣＨ５０抑制小鼠黑色素瘤的生长和转移，ＣＨ５０的抗肿瘤机制与其粘附肿瘤细胞，提高巨噬细胞杀瘤性活性有关。

Effect of recombinant human fibronectin polypeptide CH50 on growth and metastasis of melanoma.

AIM: To study the effect of recombinant human fibronectin polypeptide CH50 on murine melanoma growth and metastasis, and its antitumor mechanism. METHODS: Mouse and melanoma B16 cell tests were used to observe antitumor effect and mechanism of CH50. RESULTS: CH50 markedly inhibited melanoma growth and experimental lung metastasis. The melanoma weight was reduced from (2.3 +/- 1.2) g of control group to (0.7 +/- 0.8) g of test group (P < 0.05). CH50 at 0.125, 0.25, 0.5 mg/mouse reduced melanoma lung metastatic colonies from 87 +/- 49 of control group to 34 +/- 6, 14 +/- 12, 4 +/- 2, respectively. CH50 adhered to melanoma B16 cells and inhibited adhesion of B16 cells to laminin. CH50 enhanced the cytotoxicity of macrophages to melanoma B16 cells. CONCLUSION: CH50 inhibited tumor growth and metastasis of murine melanoma. The antitumor effect of CH50 is related to its adhesion ability to melanoma B16 cells and enhancing macrophage cytotoxicity.