| 已酮可可碱与蛋白激酶C抑制剂对佛波醇酯诱导脑微血管内皮细胞？… |
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| 引用本文： | 储智勇 黄耀诚. 已酮可可碱与蛋白激酶C抑制剂对佛波醇酯诱导脑微血管内皮细胞？…[J]. 中国药理学报, 1999, 20(8): 741-744 |
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| 作者姓名： | 储智勇 黄耀诚 |
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| 摘 要： | 目的：研究佛波醇酯（ＰＭＡ）地大鼠脑微血管内皮细胞（ＲＢＭＥＣ）表达细胞间粘附分子－１（ＩＣＡＭ－１）及ＰＫＣ抑制剂Ｈ７与已酮可可碱（ＰＴＸ）的抑制作用。方法：采用ＥＬＩＳＡ方法测定培养ＲＢＭＥＣ表达ＩＣＡＭ－１。结果：ＰＭＡ在１０－１００．Ｌ＾－１范围内剂量依赖性地诱导ＲＢＭＥＣ表达ＩＣＡＭ－１；在４－１６ｈ范围内时间依赖性诱导ＲＢＭＥＣ表达ＩＣＡＭ－１。Ｈ７和ＰＴＸ分别在５－５０μｍｏｌ．Ｌ＾
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| 关 键 词： | 血管内皮 蛋白激酶C 已酮可可碱 脑微血管 |
Effects of pentoxifylline and protein kinase C inhibitor on phorbol ester-induced intercellular adhesion molecule-1 expression in brain microvascular endothelial cells. |
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| Z Y Chu,Y C Rui. Effects of pentoxifylline and protein kinase C inhibitor on phorbol ester-induced intercellular adhesion molecule-1 expression in brain microvascular endothelial cells.[J]. Acta Pharmacologica Sinica, 1999, 20(8): 741-744 |
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| Authors: | Z Y Chu Y C Rui |
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| Affiliation: | Department of Pharmacology, College of Pharmacy, Second Military Medical University, Shanghai, China. |
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| Abstract: | AIM: To study the potential roles of protein kinase C (PKC) on expression of intercellular adhesion molecule-1 (ICAM-1) in rat brain microvascular endothelial cells (RBMEC). METHODS: ICAM-1 expression in RBMEC was measured by ELISA analyses. RESULTS: Phorbol ester (PMA) enhanced the expression of ICAM-1 in a concentration (10-100 nmol.L-1) and time (4-16 h)-dependent manner in RBMEC. Pentoxifylline (PTX) 1-100 mumol.L-1 and the PKC inhibitor H7 5-50 mumol.L-1 prevented PMA-induced stimulation of ICAM-1 expression. At PTX 100 mumol.L-1 and H7 50 mumol.L-1, they reached maximal inhibitory effects [ICAM-1 expression (A) from (0.410 +/- 0.014) to (0.175 +/- 0.022) and (0.182 +/- 0.013), respectively; P < 0.01]. CONCLUSION: Activation of PKC in RBMEC is associated with increased expression of ICAM-1 in RBMEC. PTX and H7 preincubation may inhibit PKC-induced up-regulation of ICAM-1. |
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