| 普鲁托品对兔血小板内钙的影响 |
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| 引用本文： | 沈志强 陈植和. 普鲁托品对兔血小板内钙的影响[J]. 中国药理学报, 1999, 20(4): 338-340 |
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| 作者姓名： | 沈志强 陈植和 |
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| 作者单位： | [1]云南省天然药物药理重点实验室 [2]昆明医学院第一附属医院影像学科 |
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| 摘 要： |
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| 关 键 词： | 普鲁托品 腺苷二磷酸 血小板激活因子 血小板 钙 |
Effect of protopine on cytosolic Ca2+ in rabbit platelets. |
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| Z Q Shen,Z H Chen,L Duan. Effect of protopine on cytosolic Ca2+ in rabbit platelets.[J]. Acta Pharmacologica Sinica, 1999, 20(4): 338-340 |
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| Authors: | Z Q Shen Z H Chen L Duan |
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| Affiliation: | Yunnan Pharmacological Laboratories of Natural Products, Kunming Medical College, China. |
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| Abstract: | AIM: To study the influence of protopine (Pro) on the cytoplasmic free Ca2+ concentration ([Ca2+]i) in rabbit platelets. METHODS: Measurement of [Ca2+]i of platelets in vitro by Fura 2-AM fluorescence technique. RESULTS: In the presence of CaCl2 1 mmol.L-1, Pro 10, 20, and 40 mumol.L-1 attenuated the rise in [Ca2+]i evoked by ADP from (420 +/- 57) to (320 +/- 26), (264 +/- 21), and (180 +/- 14) nmol.L-1, respectively, by arachidonic acid (AA) from (280 +/- 36) to (210 +/- 17), (184 +/- 21), and (143 +/- 16) nmol.L-1, respectively, and by platelet-activating factor (PAF) from (350 +/- 42) to (282 +/- 31), (223 +/- 30), and (165 +/- 15) nmol.L-1, respectively. In the presence of egtazic acid 1 mmol.L-1, Pro 10, 20, and 40 mumol.L-1 reduced the Ca2+ release induced by ADP, AA, and PAF, respectively. Pro 10, 20, and 40 mumol.L-1 also decreased ADP-, AA-, and PAF-induced Ca2+ influx. CONCLUSION: Pro inhibited not only Ca2+ release but also the influx of Ca2+. |
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