逍遥散对慢性心理应激损伤大鼠海马神经元的影响

目的:观测逍遥散对多相性应激大鼠海马神经元结构的影响,探究逍遥散抵抗应激损伤大鼠海马神经元结构的机制.方法:大鼠采用慢性多相性应激模型,造模后取材进行Nissl体染色观测海马神经元内Nissl体及Sevier-Munger海马神经纤维,以Fura-2负载及荧光分光光度计检测大鼠海马突触体内Ca2 浓度.结果:造模组Nissl体数量明显减少,神经元胞体溃变、减少,神经纤维减少、排列紊乱;逍遥散治疗组Nissl体及神经元胞体及神经纤维数量有明显增加,排列更整齐;海马突触体内Ca2 浓度均明显升高.结论:多相性应激使大鼠海马神经元损伤从而致神经元内Nissl体减少,这种损伤是可逆或部分可逆的,逍遥散能明显抑制应激对大鼠海马神经元造成的损伤及神经元内Nissl体的减少.海马神经元细胞内Ca2 超载可能是慢性心理应激损伤大鼠空间学习记忆重要的神经机制,逍遥散也可能是通过抑制海马神经元细胞外Ca2 大量内流,阻止Ca2 超载,从而改善应激大鼠的学习记忆状况.

Effect of Xiaoyaosan(逍遥散) on hippocampal synaptic structural plasticity of rats under multi-stress model

Objective: To observe the influence and study the mechanisms of Xiaoyaosan (XYS) to hippocampal nervous structural synaptosome of rats under multi-stress methods. Methods: Rats were randomly allocated to tive groups (normal control group, model group 1, model group 2, XYS group 1, XYS group 2). Model was selected according to Carts. Nissl body of Hippo-campal nerve-cell was detected by Nissl staining, Hippocampal synaptosome were detected by Sevier-Munger staining. The concentration of hippocampal synaptic Ca 2+ ion was detected by fluorimetry. Results: Contrasted with normal control group, Nissl bodies and hippocampal synaptosomes of model group 1 rats decreased, it became disorder that the arrangement of model groups rats hippocampal synaptosome. Those change of XYS groups was less than model groups. Concentration of Ca 2+ ion in hippocampal synaptosome of rats, normal control group is less than the other groups (P<0.01); XYS group 1 is less than both model groups (P<0.01); XYS group 2 is less than XYS group 1 (P<0.05); model group 2 is also less than model group 1 (P<0.05). Conclusion: These observations suggest that the multi-sress model could damage hippocampal synaptic structure by increasing the concentration of Ca 2+ ion in hippocampal synaptosome of rats and XYS can decrease this kind of damage by restricting the concentration of Ca 2+ ion in hippocampal synaptosome of rats increasing.